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首页> 外文期刊>Biochemistry >A Rewired Green Fluorescent Protein: Folding and Function in a Nonsequential, Noncircular GFP Permutant
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A Rewired Green Fluorescent Protein: Folding and Function in a Nonsequential, Noncircular GFP Permutant

机译：重新连接的绿色荧光蛋白：折叠和非顺序，非圆形GFP置换中的功能。

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摘要

The sequential order of secondary structural elements in proteins affects the folding and activity tonan unknown extent. To test the dependence on sequential connectivity, we reconnected secondary structuralnelements by their solvent-exposed ends, permuting their sequential order, called “rewiring”. This new proteinndesign strategy changes the topology of the backbone without changing the core side chain packing arrangement.nWhile circular and noncircular permutations have been observed in protein structures that are not related bynsequence homology, to date no one has attempted to rationally design and construct a protein with a sequencenthat is noncircularly permuted while conserving three-dimensional structure. Herein, we show that greennfluorescent protein can be rewired, still functionally fold, and exhibit wild-type fluorescence excitation andnemission spectra.
机译：蛋白质中二级结构元素的顺序影响折叠和活性至未知的程度。为了测试对顺序连接性的依赖性，我们通过溶剂暴露的末端重新连接了二级结构元件，排列了它们的顺序，称为“重排”。这种新的蛋白质设计策略可在不改变核心侧链堆积排列的情况下改变主链的拓扑结构.n虽然在与序列同源性无关的蛋白质结构中观察到了圆形和非圆形排列，但迄今为止尚未有人尝试合理设计和构建蛋白质具有在保持三维结构的同时非圆形排列的序列。在这里，我们显示绿色荧光蛋白可以被重新连接，仍然功能折叠，并表现出野生型荧光激发和发射光谱。

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  • 来源
    《Biochemistry》 |2010年第51期|p.10773-10779|共7页
  • 作者

    Philippa J. Reeder Yao-Ming Huang Jonathan S. Dordick and Christopher Bystroff;

  • 作者单位

    ‡Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, United States,§Department of Biology, and ) Department of Chemical and Biological Engineering, Center for Biotechnologyand Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, United States;

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