Contributions of Stacking, Preorganization, and Hydrogen Bonding to the Thermodynamic Stability of Duplexes between RNA and 2′-O-Methyl RNA with Locked Nucleic Acids

Elzbieta Kierzek Anna Pasternak Karol Pasternak Zofia Gdaniec Ilyas Yildirim Douglas H. Turner and Ryszard Kierzek

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Contributions of Stacking, Preorganization, and Hydrogen Bonding to the Thermodynamic Stability of Duplexes between RNA and 2′-O-Methyl RNA with Locked Nucleic Acids

机译：堆积，预组织和氢键对RNA和带有锁定核酸的2'-O-甲基RNA双链体热力学稳定性的贡献

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Locked nucleic acids (LNA) considerably enhance the thermodynamic stability ofDNAandRNAnduplexes. We report the thermodynamic stabilities of LNA-20n-O-methyl RNA/RNA duplexes designed tonprovide insight into the contributions of stacking and hydrogen bonding interactions to the enhancednstability. The results show that hydrogen bonding of LNA nucleotides is similar to that of 20n-O-methyl RNAnnucleotides, whereas the 30n-stacking interactions are on average ∼0.7 kcal/mol more favorable at 37 u0002C thannfor 20n-O-methyl or RNA nucleotides. Moreover, NMR spectra suggest helical preorganization of the single-nstranded tetramer, CLnAMALnUM, probably due to restriction of some torsion angles. Thus, enhanced stackingninteractions and helical preorganization of single-stranded oligonucleotides contribute to the extraordinarynstabilization of duplexes by LNA nucleotides

机译：锁定核酸（LNA）大大增强了DNA和RNA双链体的热力学稳定性。我们报告了设计的LNA-20n-O-甲基RNA / RNA双链体的热力学稳定性，从而提供了对堆积和氢键相互作用对增强n稳定性的贡献的见识。结果表明，LNA核苷酸的氢键与20n-O-甲基RNA核苷酸的氢键相似，而30n-堆叠的相互作用在37 u0002C时平均比nn对20n-O-甲基或RNA核苷酸更有利，约为0.7 kcal / mol。此外，NMR光谱表明单nstranded四聚体CLnAMALnUM的螺旋预组织，可能是由于某些扭转角的限制。因此，增强的堆叠链相互作用和单链寡核苷酸的螺旋预组织有助于通过LNA核苷酸使双链体异常稳定。

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《Biochemistry》 |2009年第20期|p.4377-4387|共11页
作者
Elzbieta Kierzek Anna Pasternak Karol Pasternak Zofia Gdaniec Ilyas Yildirim Douglas H. Turner and Ryszard Kierzek;
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‡Institute of Bioorganic Chemistry, Polish Academy of Sciences, 60-714 Poznan, Noskowskiego 12/14, Poland,§Department of Chemistry, University of Rochester, RC Box 270216, Rochester, New York 14627-0216, and) Department of Pediatrics, University of Rochester, School of Medicine and Dentistry, Rochester, New York 14642^Present address: Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark,Campusvej 55, DK-5230 Odense M, Denmark;

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2. Understanding the Effect of Locked Nucleic Acid and 2′-O-Methyl Modification on the Hybridization Thermodynamics of a miRNA?mRNA Pair in the Presence and Absence of AfPiwi Protein [J] . Santosh Kumar, Koyeli Mapa, Souvik Maiti Biochemistry . 2014,第10期

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3. The influence of locked nucleic acid residues on the thermodynamic properties of 2 '-O-methyl RNA/RNA heteroduplexes [J] . Kierzek E, Ciesielska A, Pasternak K, Nucleic Acids Research . 2005,第16期

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5. Structure and function of circadian clock proteins and deuterium isotope effects in nucleic acid hydrogen bonds. [D] . Vakonakis, Ioannis. 2004

机译：昼夜节律蛋白的结构和功能以及核酸氢键中的氘同位素效应。
6. Contributions of stacking preorganization and hydrogen bonding to the thermodynamic stability of duplexes between RNA and 2-O-methyl RNA with Locked Nucleic Acids (LNA) [O] . Elzbieta Kierzek, Anna Pasternak, Karol Pasternak, -1

机译：堆叠preorganization并与锁核酸RNa和2-O-甲基RNa之间双链体的热力学稳定性氢键的贡献（LNa）
7. The influence of locked nucleic acid residues on the thermodynamic properties of 2′-O-methyl RNA/RNA heteroduplexes [O] . Kierzek Elzbieta, Ciesielska Anna, Pasternak Karol, 2005

机译：锁定的核酸残基对2'-O-甲基RNA / RNA异源双链体热力学性质的影响

Contributions of Stacking, Preorganization, and Hydrogen Bonding to the Thermodynamic Stability of Duplexes between RNA and 2′-O-Methyl RNA with Locked Nucleic Acids

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