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首页> 外文期刊>Biochemistry >The RNA Recognition Mechanism of Human Immunodeficiency Virus (HIV) Type 2 NCp8 Is Different from That of HIV-1 NCp7
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The RNA Recognition Mechanism of Human Immunodeficiency Virus (HIV) Type 2 NCp8 Is Different from That of HIV-1 NCp7

机译：人类免疫缺陷病毒（HIV）2型NCp8的RNA识别机制不同于HIV-1 NCp7的RNA识别机制。

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The nucleocapsid (NC) protein of HIV, which contains two CCHC-type zinc fingersnconnected by a linker, is amultifunctional protein involved inmany of the critical steps of theHIV lifencycle. HIV-1 and HIV-2 contain NC proteins NCp7 and NCp8, respectively. The amino acidnsequences of both NC proteins are 67% identical. For NCp7, the important elements for RNAnbinding were found to be the first zinc finger flanked by the linker, as the minimal active domain, andnthe 310 helix in theN-terminus, as the secondary active domain.However, for theNCp8 counterpart innHIV-2, the mechanism for binding to viral RNA has not yet been clarified. In this study, wendeterminedNCp8’s three-dimensional structure for the first time and examined the dynamic behaviornand chemical shift perturbation as a function of the concentration of viral RNA SL3.Moreover, thenspecific binding activities ofNCp8 and theNCp8-derived peptideswith SL3were examined by a nativenpolyacrylamide gel electrophoresis assay. These results indicate that theRNArecognitionmechanismnfor NCp8 is different from that of NCp7 and that the hydrophobic cleft in the second zinc finger actsnas a secondary active domain instead of the 310 helix inNCp7. Furthermore, the flexibility of the linkernis limited by the hydrogen bond between the first zinc finger (Asn11) and the linker (Arg27), whichnmakes it possible for the sites around Trp10 in the minimal active domain and the secondary activendomain to form the binding surface.
机译：HIV的核衣壳（NC）蛋白包含两个通过接头连接的CCHC型锌指，是一种多功能蛋白，涉及HIV生命周期的许多关键步骤。 HIV-1和HIV-2分别包含NC蛋白NCp7和NCp8。两种NC蛋白的氨基酸序列相同，为67％。对于NCp7，发现RNAn结合的重要元素是连接子侧翼的第一个锌指，作为最小活性结构域，N末端的310螺旋，作为次级活性结构域。但是，对于NCp8对应物innHIV-2，与病毒RNA结合的机制尚未阐明。在这项研究中，我们首次确定了NCp8的三维结构，并检查了其动态行为和化学位移扰动与病毒RNA SL3浓度的关系。此外，还通过天然聚丙烯酰胺凝胶电泳检查了NCp8和NCp8衍生肽与SL3的特异性结合活性。分析。这些结果表明，NCp8的RNA识别机制与NCp7的不同，并且第二个锌指中的疏水性裂缝起着次级活性域的作用，而不是NCp7中的310螺旋。此外，接头的柔韧性受到第一个锌指（Asn11）和接头（Arg27）之间氢键的限制，这使得Trp10周围最小活性域和次级活性域中的位点有可能形成结合表面。

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