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首页> 外文期刊>Biochemistry >Role of OsHAL3 protein, a putative 4'-phosphopantothenoylcysteine decarboxylase in rice
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Role of OsHAL3 protein, a putative 4'-phosphopantothenoylcysteine decarboxylase in rice

机译:OsHAL3蛋白(一种推定的4'-磷酸戊二烯酰半胱氨酸脱羧酶)在水稻中的作用

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摘要

In this study, we cloned the OsHAL3 gene from rice Oryza sativa. Alignment analysis revealed that OsHAL3 has a high sequence identity to Dfp protein in Escherichia coli and AtHAL3a protein in Arabidopsis thaliana, which have 4'-phosphopantothenoylcysteine decarboxylase (PPC-DC) activity. OsHAL3 can complement mutation in the E. coli dfp gene encoding PPC-DC, so that the mutant strains with OsHAL3 can grow on rich media at 42° C and on VB minimal media at 30° C. Complementation tests with point mutations of OsHAL3 suggested that the conserved Cys176 residue of OsHAL3 is a key active-site residue. The mutant OsHAL3 G180A has a partly reduced activity. Related mRNA-level analysis showed that the OsHAL3 gene is induced by calcium pantothenate in rice. [PUBLICATION ABSTRACT]
机译:在这项研究中,我们从水稻Oryza sativa中克隆了OsHAL3基因。序列比对分析表明,OsHAL3与大肠杆菌中的Dfp蛋白和拟南芥中的AtHAL3a蛋白具有高度的序列同一性,具有4'-磷酸蒽二酰半胱氨酸脱羧酶(PPC-DC)活性。 OsHAL3可以补充编码PPC-DC的大肠杆菌dfp基因中的突变,因此具有OsHAL3的突变菌株可以在42°C的富培养基和30°C的VB最低培养基上生长。建议对OsHAL3进行点突变的互补测试OsHAL3的保守Cys176残基是关键的活性位点残基。突变体OsHAL3 G180A具有部分降低的活性。相关的mRNA水平分析表明,OsHAL3基因是由水稻泛酸钙诱导的。 [出版物摘要]

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