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首页> 外文期刊>Biochemistry >Induction of Arabidopsis gdh2 gene expression during changes in redox state of the mitochondrial respiratory chain
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Induction of Arabidopsis gdh2 gene expression during changes in redox state of the mitochondrial respiratory chain

机译：线粒体呼吸链氧化还原状态变化期间拟南芥gdh2基因表达的诱导

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摘要

Expression of the gdh2 gene encoding the α-subunit of mitochondrial glutamate dehydrogenase depends on redox state of the mitochondrial electron transport chain. Treatment of Arabidopsis thaliana cell suspension with antimycin A, a respiratory chain complex III inhibitor, resulted in an increase in gdh2 transcripts within 2 h. Inhibition of complex I by rotenone did not influence the transcript level, but treatment with potassium cyanide, a complex IV inhibitor, also increased the transcript content. Thus, gdh2 gene expression obviously responds to changes in the respiratory chain segment localized between complexes I and III. Lack of activation of gene expression after treatment of a cell suspension with hydrogen per- oxide and the prooxidant paraquat and results of experiments with antioxidants suggest that gdh2 gene expression is not associated with increased content of reactive oxygen species generated during inhibition of the electron transport chain. Protein phosphorylation by serine/threonine protein kinases is the essential step required for signal transduction into nucleus resulting in the induction of gdh2 expression. [PUBLICATION ABSTRACT]
机译：编码线粒体谷氨酸脱氢酶α-亚基的gdh2基因的表达取决于线粒体电子传输链的氧化还原状态。用呼吸链复合物III抑制剂抗霉素A处理拟南芥细胞悬液，导致2小时内gdh2转录物增加。鱼藤酮对复合物I的抑制作用不会影响转录水平，但是用氰化钾（一种复合物IV抑制剂）处理也会增加转录物的含量。因此，gdh2基因表达显然响应复合物I和III之间的呼吸链节段的变化。过氧化氢和百草枯过氧化物处理细胞悬液后缺乏基因表达的激活以及抗氧化剂的实验结果表明，gdh2基因表达与抑制电子传输链过程中产生的活性氧含量增加无关。丝氨酸/苏氨酸蛋白激酶的蛋白磷酸化是信号转导至核中，从而诱导gdh2表达所需的必要步骤。 [出版物摘要]

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