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Identification of messenger RNA substrates for mouse T-STAR

机译:小鼠T-STAR信使RNA底物的鉴定

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摘要

Using the method of isolation of specific nucleic acids associated with proteins (SNAAP), we have identified 10 candidate target mRNA substrates bound by mT-STAR (mouse T-STAR protein) from testis extract. Among them, our study focused on Fabp9, a gene that is essential for male gametogenesis, and showed that mT-STAR could directly bind to Fabp9 mRNAs. The binding sites are in a short sequence of the coding region and 3' untranslated region of Fabp9 mRNA. These suggest that mT-STAR can regulate the metabolism and expression of Fabp9. In conclusion, identification of mT-STAR-bound mRNA substrates might help to illustrate the potential spectrum of the process and provide valuable insight into the biological function of this RNA-binding protein in spermatogenesis. [PUBLICATION ABSTRACT]
机译:使用分离与蛋白质相关的特定核酸(SNAAP)的方法,我们从睾丸提取物中鉴定了10种与mT-STAR(小鼠T-STAR蛋白)结合的候选靶mRNA底物。其中,我们的研究集中于Fabp9,这是雄性配子发生必不可少的基因,并表明mT-STAR可以直接结合Fabp9 mRNA。结合位点在Fabp9 mRNA的编码区和3'非翻译区的短序列中。这些提示mT-STAR可以调节Fabp9的代谢和表达。总之,鉴定与mT-STAR结合的mRNA底物可能有助于说明该过程的潜在光谱,并提供对该RNA结合蛋白在精子发生中的生物学功能的宝贵见解。 [出版物摘要]

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