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Effects of gasoline components on MTBE and TBA cometabolism by Mycobacterium austroafricanum JOB5

机译：汽油成分对分枝杆菌JOB5对MTBE和TBA代谢的影响

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In this study we have examined the effects of individual gasoline hydrocarbons (C5–10,12,14 n-alkanes, C5–8 isoalkanes, alicyclics [cyclopentane and methylcyclopentane] and BTEX compounds [benzene, toluene, ethylbenzene, m-, o-, and p-xylene]) on cometabolism of methyl tertiary butyl ether (MTBE) and tertiary butyl alcohol (TBA) by Mycobacterium austroafricanum JOB5. All of the alkanes tested supported growth and both MTBE and TBA oxidation. Growth on C5–8 n-alkanes and isoalkanes was inhibited by acetylene whereas growth on longer chain n-alkanes was largely unaffected by this gas. However, oxidation of both MTBE and TBA by resting cells was consistently inhibited by acetylene, irrespective of the alkane used as growth-supporting substrate. A model involving two separate but co-expressed alkane-oxidizing enzyme systems is proposed to account for these observations. Cyclopentane, methylcyclopentane, benzene and ethylbenzene did not support growth but these compounds all inhibited MTBE and TBA oxidation by alkane-grown cells. In the case of benzene, the inhibition was shown to be due to competitive interactions with both MTBE and TBA. Several aromatic compounds (p-xylene > toluene > m-xylene) did support growth and cells previously grown on these substrates also oxidized MTBE and TBA. Low concentrations of toluene (<10 μM) stimulated MTBE and TBA oxidation by alkane-grown cells whereas higher concentrations were inhibitory. The effects of acetylene suggest strain JOB5 also has two distinct toluene-oxidizing activities. These results have been discussed in terms of their impact on our understanding of MTBE and TBA cometabolism and the enzymes involved in these processes in mycobacteria and other bacteria.
机译：在这项研究中，我们研究了单个汽油烃（C 5–10,12,14 正构烷烃，C 5–8 异烷烃，脂环族[环戊烷和甲基环戊烷]和BTEX化合物[苯，甲苯，乙苯，间，邻和对二甲苯]）通过奥古杆菌分枝杆菌JOB5代谢甲基叔丁基醚（MTBE）和叔丁醇（TBA）。所有测试的烷烃均支持生长以及MTBE和TBA氧化。乙炔可抑制C 5-8 正构烷烃和异烷烃的生长，而长链正构烷烃的生长在很大程度上不受该气体的影响。但是，无论烷烃用作生长支持底物，乙炔始终抑制静止细胞对MTBE和TBA的氧化。提出了一个模型，该模型涉及两个单独但共表达的烷烃氧化酶系统，以解释这些观察结果。环戊烷，甲基环戊烷，苯和乙苯不支持生长，但是这些化合物均抑制了烷烃生长细胞的MTBE和TBA氧化。对于苯而言，抑制作用被证明是由于与MTBE和TBA的竞争性相互作用。几种芳族化合物（对二甲苯>甲苯>间二甲苯）确实支持了生长，先前在这些底物上生长的细胞也氧化了MTBE和TBA。低浓度的甲苯（<10μM）会刺激烷烃生长细胞的MTBE和TBA氧化，而较高的浓度则具有抑制作用。乙炔的影响表明，菌株JOB5还具有两种不同的甲苯氧化活性。已就它们对我们对MTBE和TBA代谢的理解以及分枝杆菌和其他细菌中与这些过程有关的酶的影响进行了讨论，讨论了这些结果。

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