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首页> 外文期刊>Bioenergy research >A Novel Aldehyde Reductase Encoded by YML131W from Saccharomyces cerevisiae Confers Tolerance to Furfural Derived from Lignocellulosic Biomass Conversion
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A Novel Aldehyde Reductase Encoded by YML131W from Saccharomyces cerevisiae Confers Tolerance to Furfural Derived from Lignocellulosic Biomass Conversion

机译：由酿酒酵母YML131W编码的新型醛还原酶赋予对木质纤维素生物质转化衍生的糠醛耐受性

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摘要

Furfural is one of the main aldehyde inhibitors generated during the pretreatment of lignocellulosic biomass. Saccharomyces cerevisiae can in situ detoxify furfural to the less toxic furan methanol (FM) via the activities of multiple dehydrogenases/reductases. In this study, we report that an uncharacterized gene, YML131W, was highly induced under furfural stress conditions and that the transcription factors Yap1p, Msn2/4p, and/or Hsf1p likely controlled its upregulated expression. The induced transcription of YML131W led to higher concentrations of its encoded protein. Enzyme activity assays showed that YML131W is an aldehyde reductase that plays a role in detoxifying furfural to FM. YML131W also showed activity toward other aldehydes, suggesting that it is involved in detoxifying endogenous toxic aldehydes generated via the degradation of misfolded and damaged proteins. This detoxification role would help to maintain cell viability under furfural stress conditions. A S. cerevisiae strain overexpressing YML131W showed increased tolerance to furfural. YML131W was able to catalyze the conversion of formaldehye, acetaldehyde, propionaldehyde, and butyaldehyde to their corresponding alcohols, indicating that it has potential applications in producing fuels such as butanol and isobutanol. A phylogenetic analysis grouped YML131W into the leukotriene B4 dehydrogenases (LTD) family, but its amino acid sequence substantially differed from those of other proteins in the LTD family. We identified 15 proteins from 14 yeast species that showed sequence similarities to YML131W. These other proteins likely have similar functions to that of YML131W and may have potential to confer tolerance to aldehyde inhibitors derived from the lignocellulosic biomass conversion process.
机译：糠醛是木质纤维素生物质预处理过程中产生的主要醛类抑制剂之一。酿酒酵母可通过多种脱氢酶/还原酶的活性原位将糠醛解毒成毒性较小的呋喃甲醇（FM）。在这项研究中，我们报道了在糠醛胁迫条件下高度诱导的未鉴定基因YML131W，转录因子Yap1p，Msn2 / 4p和/或Hsf1p可能控制了其上调表达。 YML131W的诱导转录导致其编码蛋白的浓度更高。酶活性测定表明，YML131W是一种醛还原酶，在糠醛对FM的解毒中起作用。 YML131W还显示出对其他醛的活性，表明它参与了对由于折叠错误和受损蛋白质的降解而产生的内源性有毒醛进行解毒的工作。这种解毒作用将有助于在糠醛胁迫条件下维持细胞活力。过量表达YML131W的酿酒酵母菌株显示出对糠醛的耐受性增加。 YML131W能够催化甲醛，乙醛，丙醛和丁醛转化为相应的醇，这表明它在生产燃料（例如丁醇和异丁醇）方面具有潜在的应用前景。系统发育分析将YML131W分为白三烯B4脱氢酶（LTD）家族，但其氨基酸序列与LTD家族中其他蛋白质的氨基酸序列实质上不同。我们从14种酵母中鉴定了15种蛋白质，这些蛋白质显示出与YML131W的序列相似性。这些其他蛋白质可能具有与YML131W相似的功能，并且可能具有对源自木质纤维素生物质转化过程的醛类抑制剂的耐受性。

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  • 来源
    《Bioenergy research》 |2015年第1期|119-129|共11页
  • 作者

    Xi Li; Ruoheng Yang; Menggen Ma; Xu Wang; Juan Tang; Xianxian Zhao; Xiaoping Zhang;

  • 作者单位

    1.College of Landscape Architecture Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China;

    2.College of Agronomy Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China;

    3.Institute of Ecological and Environmental Sciences College of Resource and Environmental Sciences Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China 4.Department of Applied Microbiology College of Resourceand Environmental Sciences Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China;

    4.Department of Applied Microbiology College of Resourceand Environmental Sciences Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China;

    2.College of Agronomy Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China;

    3.Institute of Ecological and Environmental Sciences College of Resource and Environmental Sciences Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China;

    4.Department of Applied Microbiology College of Resourceand Environmental Sciences Sichuan Agricultural University No. 211 Huimin Road Wenjiang 611130 Sichuan People’s Republic of China;

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  • 正文语种 eng
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  • 关键词

    Aldehyde reductase Detoxification Ethanol Furfural Lignocellulosic biomass Saccharomyces cerevisiae;

    机译：乙醛还原酶排毒乙醇糠醛木质纤维素生物质酿酒酵母;

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