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Effect of Hot-Pressing Temperature on the Subsequent Enzymatic Saccharification and Fermentation Performance of SPORL Pretreated Forest Biomass

机译:热压温度对SPORL预处理森林生物质后续酶促糖化和发酵性能的影响

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摘要

Methods to increase the energy density of biofuel feedstock for shipment are important towards improving supply chain efficiency in upstream processes. Towards this end, densified pretreated lignocellulosic biomass was produced using hot-pressing. The effects of fiber hornification induced by hot-pressing on enzymatic digestibilities of lodgepole pine and poplar NE222 wood chips pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) were examined. Pretreated wood chips were pressed at 25, 70, 90, 110, and 177 °C. The cellulose accessibilities of the pressed and unpressed substrates were evaluated using water retention value and direct cellulase adsorption measurements. Hot-pressing below 110 °C produced a degree of hornification (DH) below 0.26 and had limited effect on cellulose accessibility and enzymatic digestibility. Hot-pressing at 177 °C produced a DH of 0.86 that substantially hornified the fibers and resulted near zero saccharification. The saccharification results were consistent with cellulose accessibility data. Ethanol fermentation studies at 18 % solids suggest that a pressing below 110 °C is preferred to reduce its effect on biofuel yield.
机译:增加运输用生物燃料原料的能量密度的方法对于提高上游流程的供应链效率非常重要。为此,使用热压生产致密的预处理木质纤维素生物质。研究了热压诱导的纤维角质化对亚硫酸盐预处理以克服木质纤维素(SPORL)难降解性的黑松和杨树NE222木片的酶消化率的影响。将预处理过的木片在25、70、90、110和177°C下压制。使用保水值和直接纤维素酶吸附测量值评估压制和未压制基材的纤维素可及性。在110°C以下热压产生的角化度(DH)低于0.26,并且对纤维素可及性和酶消化率的影响有限。在177°C的温度下热压可产生0.86的DH,使纤维基本成角,糖化几乎为零。糖化结果与纤维素可及性数据一致。乙醇发酵的固体含量为18%,建议将压力降至110°C以下是首选方法,以降低其对生物燃料产量的影响。

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