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Characterization of the Interactions between Polyethylene Glycol and Cellulase during the Hydrolysis of Lignocellulose

机译：木质纤维素水解过程中聚乙二醇与纤维素酶相互作用的表征

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摘要

The interaction between polyethylene glycol (PEG) and the substrate has been widely examined for the purpose of using it to improve the enzymatic hydrolysis efficiency of lignocellulosic biomass. However, the knowledge about the positive effect of PEG on cellulase remains very limited and the mechanism underlying this positive effect has not been clearly elucidated. Our study found that the conversion rates of corn stover and Avicel PH101 increased to 53.8 and 86.8 %, respectively, with the pretreatment using PEG 6000 prior to hydrolysis. After incubation for 48 h, 68.2 % of the enzymatic activity was retained in the solution, whereas in the control, only 38.6 % of the enzyme activity was retained. The existence of this interaction and entropy generation was qualitatively confirmed by calorimetric titration. Moreover, entropy was identified as a reason for the hydrolytic acceleration. Additionally, hydrophobic effects and the hydrogen bonds formed between PEG and key amino acids contributed to cellulase activity enhancement. These results provide insight into the observed positive effects of PEG on cellulase function and indicate that PEG pretreatment would be beneficial to reduce enzyme load.
机译：聚乙二醇（PEG）和底物之间的相互作用已得到广泛研究，目的是利用它提高木质纤维素生物质的酶促水解效率。然而，关于PEG对纤维素酶的正面作用的知识仍然非常有限，并且尚未明确阐明这种正面作用的机制。我们的研究发现，在水解之前使用PEG 6000进行预处理，玉米秸秆和Avicel PH101的转化率分别提高至53.8和86.8％。孵育48小时后，溶液中保留了68.2％的酶活性，而在对照中仅保留了38.6％的酶活性。通过量热滴定定性地证实了这种相互作用和熵产生的存在。此外，熵被认为是水解加速的原因。另外，疏水作用和PEG与关键氨基酸之间形成的氢键有助于增强纤维素酶活性。这些结果提供了对观察到的PEG对纤维素酶功能的积极作用的了解，并表明PEG预处理将有利于降低酶负荷。

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  • 来源
    《Bioenergy research》 |2015年第1期|270-278|共9页
  • 作者

    Zhiyou Zong; Lijuan Ma; Liang Yu; Dongyuan Zhang; Zhenhua Yang; Shulin Chen;

  • 作者单位

    1.Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin China;

    1.Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin China;

    2.Department of Biological Systems Engineering Washington State University Pullman WA 99164 USA;

    1.Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin China;

    1.Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin China;

    1.Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences Tianjin China;

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  • 正文语种 eng
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  • 关键词

    Lignocellulose Cellulase Polyethylene glycol Hydrolysis Entropy generation Hydrogen bonds;

    机译：木质纤维素纤维素酶聚乙二醇水解熵产生氢键;

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