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首页> 外文期刊>Bioenergy research >Comparison of Dilute Acid and Sulfite Pretreatment for Enzymatic Saccharification of Earlywood and Latewood of Douglas fir
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Comparison of Dilute Acid and Sulfite Pretreatment for Enzymatic Saccharification of Earlywood and Latewood of Douglas fir

机译：酸和亚硫酸盐预处理对花旗松早木和晚木酶促糖化的比较

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摘要

This study applied dilute acid (DA) and sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL) to deconstruct earlywood and latewood cell walls of Douglas fir for fermentable sugars production through subsequent enzymatic hydrolysis. DA pretreatment removed almost all the hemicelluloses, while SPORL at initial pH = 4.5 (SP-B) removed significant amount of lignin between 20 and 25 %. But both are not sufficient for effective enzymatic saccharification. SPORL at low initial pH = 2 (SP-AB) combines the advantage of both DA and SPORL-B to achieve approximately 90 % hemicellulose removal and delignification of 10–20 %. As a result, SP-AB effectively removed recalcitrance and thereby significantly improved enzymatic saccharification compared with DA and SP-B. Results also showed that earlywood with significantly lower density produced less saccharification after DA pretreatment, suggesting that wood density does not contribute to recalcitrance. The thick cell wall of latewood did not limit chemical penetration in pretreatments. The high lignin content of earlywood limited the effectiveness of DA pretreatment for enzymatic saccharification, while hemicellulose limits the effectiveness of high pH pretreatment of SP-B. The higher hemicellulose content in the earlywood and latewood of heartwood reduced saccharification relative to the corresponding earlywood and latewood in the sapwood using DA and SP-AB.
机译：这项研究应用稀酸（DA）和亚硫酸盐预处理来克服木质纤维素（SPORL）的顽固性，以解构花旗松的早木和晚木细胞壁，以通过随后的酶促水解生产可发酵的糖。 DA预处理去除了几乎所有的半纤维素，而SPORL在初始pH == 4.5（SP-B）时去除了20％至25％之间的大量木质素。但是两者都不足以有效地进行酶促糖化。低初始pH = 2（SP-AB）时的SPORL结合了DA和SPORL-B的优势，实现了约90％的半纤维素去除和10-20％的去木质素。结果，与DA和SP-B相比，SP-AB有效地消除了顽固性，从而显着改善了酶促糖化作用。结果还表明，密度显着较低的早材在DA预处理后产生的糖化较少，这表明木材密度不会导致顽固性。胶合板厚的细胞壁在预处理过程中不限制化学物质的渗透。早材中木质素含量高限制了DA预处理对酶促糖化的有效性，而半纤维素限制了SP-B的高pH预处理的有效性。与使用DA和SP-AB的边材中相应的早材和晚材相比，心材的早材和晚材中较高的半纤维素含量降低了糖化作用。

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  • 来源
    《Bioenergy research》 |2014年第1期|362-370|共9页
  • 作者

    Chao Zhang; Xiaochun Lei; C. Tim Scott; J.Y. Zhu; Kecheng Li;

  • 作者单位

    1.School of Chemical Engineering and Technology Tianjin University Tianjin China 2.USDA Forest Service Forest Products Laboratory Madison WI USA;

    3.Dept. of Chemical Engineering University of New Brunswick Fredericton NB Canada;

    2.USDA Forest Service Forest Products Laboratory Madison WI USA;

    2.USDA Forest Service Forest Products Laboratory Madison WI USA;

    3.Dept. of Chemical Engineering University of New Brunswick Fredericton NB Canada;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cell walls Pretreatment Enzymatic hydrolysis, earlywood and latewood Recalcitrance;

    机译：细胞壁预处理酶促水解;早木和晚木;

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