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首页> 外文期刊>Bioenergy research >Biomass and Total Lipid Content Assessment of Microalgal Cultures Using Near and Short Wave Infrared Spectroscopy
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Biomass and Total Lipid Content Assessment of Microalgal Cultures Using Near and Short Wave Infrared Spectroscopy

机译：近海和短波红外光谱法评估微藻培养物的生物量和总脂质含量

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摘要

The technique of near and short wave near-infrared spectroscopy was assessed with respect to analysis of dry matter and lipid content of microalgae with potential for biodiesel production. Microalgal culture samples were filtered through GF/C filter papers and spectral measurements of wet and oven dried (60 °C overnight) filter papers over the ranges of 300–1,100 nm and 1,100–2,500 nm were recorded. Partial least square models on culture biomass and lipid content for combined species data were poor in terms of RMSECV, RCV and the ratio of RMSECV to SD. A single species model for C. vulgaris based on 1,100–2,500 nm spectra of dry filtrate supported a model with RMSECV, RCV and SDR values of 0.32 g L−1, 0.955 and 3.38 for biomass and 0.089 g L−1, 0.874 and 2.06 with lipid, respectively. However, the dry filtrate models on biomass and lipid content performed poorly in the prediction of samples drawn from an independent series of C. vulgaris cultured under N-, P- and Fe-limited growth trial. Thus, while the near-infrared spectroscopy technique has potential for assessment of dry matter and lipid content of microalgal cultures using a dried filtrate sample, further work is required to examine the limits to model robustness.
机译：在分析具有生物柴油生产潜力的微藻干物质和脂质含量方面，评估了近波和短波近红外光谱技术。微藻培养物样品通过GF / C滤纸过滤，并记录了300–1,100 nm和1,100–2,500 nm范围内的湿法和烘箱干燥（60°C过夜）滤纸的光谱测量结果。就组合物种数据而言，关于培养生物量和脂质含量的偏最小二乘模型在RMSECV，RCV和RMSECV与SD之比方面较差。基于1,100–2,500 nm干滤液光谱的寻常型梭菌的单物种模型支持一个模型，其中生物量的RMSECV，RCV和SDR值分别为0.32 g L-1、0.955和3.38,0.089 g L-1、0.874和2.06与脂质。但是，在预测由N，P和Fe有限的生长试验培养的独立系列寻常小球藻提取的样品的预测中，有关生物质和脂质含量的干滤液模型表现不佳。因此，尽管近红外光谱技术具有使用干燥的滤液样品评估微藻培养物中干物质和脂质含量的潜力，但仍需要进一步的工作来检查对鲁棒性进行建模的极限。

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