• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioenergy research >The Role of Polymeric Micelles on Chemical Changes of Pretreated Corn Stover, Cellulase Structure, and Adsorption
【24h】

The Role of Polymeric Micelles on Chemical Changes of Pretreated Corn Stover, Cellulase Structure, and Adsorption

机译：聚合物胶束对预处理玉米秸秆化学变化，纤维素酶结构和吸附作用的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Enzymatic hydrolysis of lignocellulosic biomass is limited by rapid cellulase deactivation, consequently requiring large amounts of enzyme to maintain acceptable biomass conversion. In this study, a new approach to improve lignocellulose hydrolysis was investigated. Performing enzymatic hydrolysis of corn stover (CS) in the presence of polymeric–surfactant micelles (PMs) was demonstrated to improve hydrolysis yield to a greater extent than using only surfactant micelles. Application of 2 % (w/w) of polyethylene glycol (PEG 6000) with casein, Tween-20, and Triton X-100 at levels above the critical micelle concentrations increased the hydrolysis yield of CS containing high-bound lignin (extrusion-pretreated) by up to 87.8, 11.7, and 7.5 %, respectively. These PMs were not effective during enzymatic hydrolysis of biomass lacking lignin (Avicel) or alkali-pretreated CS (7.2 % lignin). The main reasons for the enhanced cellulase activity observed due to PEG-casein, PEG-Tween, and PEG-Triton were enhanced cellulase solubilization; reformation of α-helix substructure; and combination of induced cellulase solubilization, α-helix reformation, and chemical changes in the microstructure of biomass, respectively. Deformation of the cellulase substructure during hydrolysis of biomass and its subsequent reformation in the presence of surfactants were shown in this study for the first time. Chemical changes in the microstructure of biomass (e.g., lignin side changes, C–O bonds, and amorphous cellulose) were found to be another potential reason for the effectiveness of surfactants when they are incubated at above 6 g/L for 72 h with biomass.
机译：木质纤维素生物质的酶促水解受到纤维素酶快速失活的限制，因此需要大量酶来维持可接受的生物质转化。在这项研究中，研究了一种改善木质纤维素水解的新方法。与仅使用表面活性剂胶束相比，在聚合物-表面活性剂胶束（PMs）存在下进行玉米秸秆（CS）的酶水解可显着提高水解产量。以2％（w / w）的聚乙二醇（PEG 6000）和酪蛋白，吐温20和Triton X-100的含量在临界胶束浓度以上使用可提高CS的高结合木质素的水解产率（挤压预处理） ）分别最多增加87​​.8％，11.7和7.5％。这些PM在缺乏木质素（Avicel）或碱预处理过的CS（7.2％木质素）的生物质的酶促水解过程中无效。 PEG-酪蛋白，PEG-Tween和PEG-Triton引起的纤维素酶活性增强的主要原因是纤维素酶的增溶作用。 α-螺旋亚结构的重整；分别与诱导纤维素酶溶解，α-螺旋重整和生物质微观结构的化学变化相结合。这项研究首次显示了在生物质水解过程中纤维素酶亚结构的变形以及随后在表面活性剂存在下的重整。当将表面活性剂与生物质在6 g / L以上孵育72小时时，发现生物质微观结构中的化学变化（例如，木质素侧变，C–O键和无定形纤维素）是表面活性剂有效性的另一个潜在原因。 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号