• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioenergy research >Directed Evolution of Clostridium phytofermentans Glycoside Hydrolase Family 9 Endoglucanase for Enhanced Specific Activity on Solid Cellulosic Substrate
【24h】

Directed Evolution of Clostridium phytofermentans Glycoside Hydrolase Family 9 Endoglucanase for Enhanced Specific Activity on Solid Cellulosic Substrate

机译：定向进化的Clostridium phytofermentans糖苷水解酶家族9内切葡聚糖酶增强固体纤维素底物上的比活性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Increasing specific activity of cellulase on solid cellulosic materials would be among the top priorities for second-generation biorefineries. However, the complicated relationship among the heterogeneity of solid cellulosic materials and different action mode cellulase components results in great challenges in cellulase engineering. We applied directed evolution to a Clostridium phytofermentans ISDg glycoside hydrolase family 9 processive endoglucanase (CpCel9) for enhanced hydrolytic performance by using Bacillus subtilis as a host for cloning and expression. Several CpCel9 mutants with both increased expression level and enhanced specific activity on the solid cellulosic material were obtained. The most active mutant, which also exhibits an increased expression level, had more than threefold specific activity than that of wild type on regenerated amorphous cellulose. Most mutation sites were located in the family 3 cellulose-binding module near to its catalytic module, which might guide the entrance of glucan into the catalytic module. This study suggested that directed evolution by combining B. subtilis secretory protein expression host and solid cellulosic substrates would be a powerful tool to evolve more active cellulase mutants for cost-effective biosaccharification process.
机译：纤维素酶对固体纤维素材料的比活性的提高将是第二代生物精炼厂的重中之重。然而，固体纤维素材料的异质性与不同作用方式的纤维素酶组分之间的复杂关系导致纤维素酶工程学的巨大挑战。我们应用定向进化法对Clostridium phytofermentans ISDg糖苷水解酶家族9进行性内切葡聚糖酶（CpCel9）进行了增强，通过使用枯草芽孢杆菌作为克隆和表达宿主提高了水解性能。获得了在固体纤维素材料上具有增加的表达水平和增强的比活性的几个CpCel9突变体。在再生的无定形纤维素上，活性最高的突变体（也表现出增加的表达水平）比野生型具有比野生型高三倍的比活性。大多数突变位点位于3族纤维素结合模块中，靠近其催化模块，这可能会引导葡聚糖进入催化模块。这项研究表明，通过结合枯草芽孢杆菌分泌蛋白表达宿主和固体纤维素底物进行定向进化，将成为开发更具活性的纤维素酶突变体的有效工具，以实现经济高效的生物糖化过程。

著录项

  • 来源
    《Bioenergy research》 |2014年第1期|381-388|共8页
  • 作者

    Sajjad Ahmad; Hui Ma; Muhammad Waheed Akhtar; Yi-Heng Percival Zhang; Xiao-Zhou Zhang;

  • 作者单位

    1.Department of Biological Systems Engineering Virginia Polytechnic Institute and State University Blacksburg VA 24061 USA 2.School of Biological Sciences University of the Punjab Lahore 54590 Pakistan;

    3.Gate Fuels Inc. 2200 Kraft Drive Suite 1200B Blacksburg VA 24060 USA;

    2.School of Biological Sciences University of the Punjab Lahore 54590 Pakistan;

    1.Department of Biological Systems Engineering Virginia Polytechnic Institute and State University Blacksburg VA 24061 USA 3.Gate Fuels Inc. 2200 Kraft Drive Suite 1200B Blacksburg VA 24060 USA 4.Institute for Critical Technology and Applied Science Virginia Polytechnic Institute and State University Blacksburg VA 24061 USA;

    1.Department of Biological Systems Engineering Virginia Polytechnic Institute and State University Blacksburg VA 24061 USA 3.Gate Fuels Inc. 2200 Kraft Drive Suite 1200B Blacksburg VA 24060 USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacillus subtilis Clostridium phytofermentans Directed evolution Endoglucanase Glycoside hydrolase family 9 Cellulose;

    机译：枯草芽孢杆菌枯草芽孢杆菌定向进化内切葡聚糖酶糖苷水解酶家族9纤维素;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号