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首页> 外文期刊>Bioenergy research >An Improved Tissue Culture System for Embryogenic Callus Production and Plant Regeneration in Switchgrass (Panicum virgatum L.)
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An Improved Tissue Culture System for Embryogenic Callus Production and Plant Regeneration in Switchgrass (Panicum virgatum L.)

机译：改良的柳枝Emb愈伤组织生产和植株再生的组织培养系统

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摘要

The increased emphasis on research of dedicated biomass and biofuel crops begs for biotechnology method improvements. For switchgrass (Panicum virgatum L.), one limitation is inefficient tissue culture and transformation systems. The objectives of this study were to investigate the utility of a new medium described here, LP9, for the production and maintenance of switchgrass callus and its regeneration, which also enables genetic transformation. LP9 medium is not based on Murashige and Skoog (MS) medium, the basal medium that all published switchgrass transformation has been performed. We demonstrate an efficient tissue culture system for switchgrass Alamo 2, which yields increased viability of callus and the ability to maintain callus for a duration of over 6 months. This longevity gives a greater useful callus lifetime than for published switchgrass MS-based media. This increased longevity enables greater potential efficiency and throughput for a transformation pipeline. Callus produced on LP9 is categorized as type II callus, which is more friable and easier to multiply, maintain and transfer than type I callus obtained from previously described tissue culture systems.
机译：对专用生物质和生物燃料作物的研究日益受到重视，以求改进生物技术方法。对于柳枝（（Panicum virgatum L.），一个局限性是效率低下的组织培养和转化系统。这项研究的目的是调查这里描述的一种新培养基LP9在柳枝call愈伤组织的产生和维持及其再生中的效用，后者还可以进行遗传转化。 LP9培养基不是基于Murashige和Skoog（MS）培养基，所有已发表的柳枝transformation转化均已进行过的基础培养基。我们展示了柳枝柳Alamo 2的有效组织培养系统，该系统可提高愈伤组织的活力并保持愈伤组织6个月以上的能力。与已发布的基于柳枝MS的基于MS的媒体相比，这种长寿可以延长其愈伤组织的使用寿命。更长的使用寿命可以为转换管道带来更大的潜在效率和吞吐量。在LP9上产生的愈伤组织被归为II型愈伤组织，与从先前描述的组织培养系统获得的I型愈伤组织相比，它更易碎，并且易于繁殖，维持和转移。

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  • 来源
    《Bioenergy research》 |2009年第4期|267-274|共8页
  • 作者

    Jason N. Burris; David G. J. Mann; Blake L. Joyce; C. Neal Stewart Jr.;

  • 作者单位

    Department of Plant Sciences, The University of Tennessee, 252 Ellington Plant Sciences, 2431 Joe Johnson Drive, Knoxville, TN 37996-4561, USA;

    Department of Plant Sciences, The University of Tennessee, 252 Ellington Plant Sciences, 2431 Joe Johnson Drive, Knoxville, TN 37996-4561, USA;

    Department of Plant Sciences, The University of Tennessee, 252 Ellington Plant Sciences, 2431 Joe Johnson Drive, Knoxville, TN 37996-4561, USA;

    Department of Plant Sciences, The University of Tennessee, 252 Ellington Plant Sciences, 2431 Joe Johnson Drive, Knoxville, TN 37996-4561, USA;

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  • 正文语种 eng
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  • 关键词

    biotechnology; bioenergy feedstock; biofuels; callus; media; somatic embryogenesis; tissue culture; transformation;

    机译：生物技术生物能源原料生物燃料愈伤组织媒体;体细胞胚发生组织培养;转型;

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