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首页> 外文期刊>Bioenergy research >High-throughput Screening of EMS Mutagenized Maize for Altered Starch Digestibility
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High-throughput Screening of EMS Mutagenized Maize for Altered Starch Digestibility

机译：高通量筛选诱变的EMS玉米淀粉

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摘要

Carbohydrate research increasingly is focused on changing the biochemical nature of starch to create more efficient substrates for biofuel production; parallel work is aimed at healthier foods for human consumption. A key factor in both of these efforts is the rate at which starch is digested by amylases. Starch digestibility is influenced heavily by genetically controlled factors including starch granular and molecular structure and composition. Maize mutant varieties with increased starch digestibility would help to make more cost-efficient biofuels. To identify such mutants among segregating families of ethyl methane sulfonate-mutagenized maize, we developed a miniaturized, high-throughput single kernel preparation and starch digestion assay that can process over 500 samples per week. In a preliminary screen of 480 families, we have identified 62 mutants with faster rates of digestion as compared to wild type and, in the same screen, an additional 53 lines with slower rates of digestion, thus tremendous potential health benefits. These mutants can be used for detailed structural analysis of starch and flour physical and chemical properties, factors that interact with starch in the cell and analysis of an apparently large number of genes that can impact rates of starch digestion.
机译：碳水化合物的研究越来越集中在改变淀粉的生化性质上，以创造出更有效的生物燃料生产底物。平行工作的目的是为人类食用更健康的食品。这两项工作的关键因素是淀粉被淀粉酶消化的速率。淀粉的消化率受遗传控制因素的影响很大，包括淀粉颗粒，分子结构和组成。具有提高的淀粉消化率的玉米突变体品种将有助于生产更具成本效益的生物燃料。为了在甲烷磺酸乙酯诱变的玉米分离家庭中鉴定此类突变体，我们开发了一种小型化，高通量的单仁制备和淀粉消化测定法，每周可处理500多个样品。在480个家庭的初步筛选中，我们鉴定出62个突变体与野生型相比具有更快的消化速率，并且在同一筛选中，还鉴定了另外53个消化速率较慢的突变体，因此具有巨大的潜在健康益处。这些突变体可用于淀粉和面粉的物理和化学特性的详细结构分析，与细胞中淀粉相互作用的因子以及可影响淀粉消化率的大量基因的分析。

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  • 来源
    《Bioenergy research》 |2008年第2期|118-135|共18页
  • 作者

    Deborah Groth; Judy Santini; Bruce Hamaker; Clifford Weil;

  • 作者单位

    1.Department of Agronomy Purdue University West Lafayette IN 47907 USA 2.Whistler Center for Carbohydrate Research Purdue University 915 W State St. West Lafayette IN 47907 USA;

    1.Department of Agronomy Purdue University West Lafayette IN 47907 USA;

    2.Whistler Center for Carbohydrate Research Purdue University 915 W State St. West Lafayette IN 47907 USA 3.Food Science Department Purdue University West Lafayette IN 47907 USA;

    1.Department of Agronomy Purdue University West Lafayette IN 47907 USA 2.Whistler Center for Carbohydrate Research Purdue University 915 W State St. West Lafayette IN 47907 USA;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Rapidly digesting starch Slowly digesting starch Resistant starch Ethanol feedstock High-throughput screen;

    机译：快速消化淀粉慢消化淀粉抗性淀粉乙醇原料高通量筛选;

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