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The application of bacteriophage to control Cronobacter sakazakii planktonic and biofilm growth in infant formula milk

机译:噬菌体在婴幼儿配方牛奶中控制茅草杆菌和生物膜生长的施用

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摘要

The goal was to identify the biofilm-forming ability of Cronobacter sakazakii on surfaces of stainless steel (SS) and silicone rubber (SR) in contact with infant formula milk. Two representative bacteriophages (PBES04 and PBES19) were used to control the growth of C. sakazakii as well as its biofilm forming ability on either SS or SR surfaces. Bacterial growth was confirmed at 20 degrees C when PBES04 and PBES19 were used, whereas C. sakazakii was not normally detected in infant formula milk treated with both bacteriophages for 6 h. In an additional biofilm reduction experiment, the biofilm on SS or SR surfaces were reduced by 3.07 and 1.92 log CFU cm(-2), respectively after PBES04 treatment, and 3.06 and 2.14 log CFU cm(-2), respectively, after PBES19 treatment. These results demonstrate that bacteriophages can be effective in inactivating C. sakazakii in biofilms which could potentially increase food safety in commercial facilities.
机译:目标是鉴定与婴儿配方牛奶接触的不锈钢(SS)和硅橡胶(SR)表面上的金刚杆菌的生物膜形成能力。 使用两个代表性噬菌体(PBES04和PBE19)来控制C.Sakazakii的生长以及Ss或Sr表面上的生物膜形成能力。 当使用PBES04和PBE19时,在20℃下确认细菌生长,而C.Sakazakii通常在用两种噬菌体治疗的婴儿配方牛奶中检测到6小时。 在额外的生物膜还原实验中,PBES04处理后,SS或Sr表面上的生物膜分别减少了3.07和1.92 log Cfu cm(-2),分别在PBES19处理后分别为3.06和2.14 Log Cfu cm(-2) 。 这些结果表明,噬菌体可以有效地在生物膜中灭活C.Sakazakii,这可能会增加商业设施的食品安全。

著录项

  • 来源
    《Biofouling》 |2021年第6期|606-614|共9页
  • 作者单位

    Chung Ang Univ Adv Food Safety Res Grp Dept Food Sci & Technol BrainKorea21 Plus Seoul South Korea;

    Chung Ang Univ Adv Food Safety Res Grp Dept Food Sci & Technol BrainKorea21 Plus Seoul South Korea;

    Chung Ang Univ Adv Food Safety Res Grp Dept Food Sci & Technol BrainKorea21 Plus Seoul South Korea;

    Chung Ang Univ Adv Food Safety Res Grp Dept Food Sci & Technol BrainKorea21 Plus Seoul South Korea;

    Chung Ang Univ Adv Food Safety Res Grp Dept Food Sci & Technol BrainKorea21 Plus Seoul South Korea;

    Livestock Serv Dhaka Bangladesh;

    Oregon State Univ Dept Food Sci & Technol Corvallis OR 97331 USA;

    Chung Ang Univ Adv Food Safety Res Grp Dept Food Sci & Technol BrainKorea21 Plus Seoul South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cronobacter sakazakii; biofilm; bacteriophage; infant formula milk;

    机译:cr s茅草饼;生物膜;噬菌体;婴儿配方牛奶;

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