Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp chinensis) cv. Lastochka

Shumilina D. V.; Shmykova N. A.; Bondareva L. L.; Suprunova T. P.

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Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp chinensis) cv. Lastochka

机译：基因型和培养基含量对小白菜小孢子来源胚形成的影响拉斯托奇卡

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The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2-2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige-Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3 g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants.

机译：研究了不同因素对大白菜小孢子胚胎发生的影响。观察到胚胎形成的基因型依赖性。大多数胚芽和植物均从从花蕾中分离出来的小孢子（长度为2-2.9毫米）中获得，并在NLN液体培养基中培养，其中添加了13％蔗糖（w / v），24-表油菜素内酯和1％活性炭。在含有2％蔗糖（w / v），0.1 mg / L苄基氨基嘌呤和3 g / L Phytagel的1/2 Murashige-Skoog培养基上培养的胚刺激了次级胚的形成，导致大量双倍单倍体发育植物。

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《Biology Bulletin》 |2015年第4期|302-309|共8页
作者
Shumilina D. V.; Shmykova N. A.; Bondareva L. L.; Suprunova T. P.;
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All Russia Res Inst Vegetable Breeding & Seed Pro, P VNIISSOK, Odintsovo Dist 143080, Moscow Region, Russia;

All Russia Res Inst Vegetable Breeding & Seed Pro, P VNIISSOK, Odintsovo Dist 143080, Moscow Region, Russia;

All Russia Res Inst Vegetable Breeding & Seed Pro, P VNIISSOK, Odintsovo Dist 143080, Moscow Region, Russia;

All Russia Res Inst Vegetable Breeding & Seed Pro, P VNIISSOK, Odintsovo Dist 143080, Moscow Region, Russia;

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Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp chinensis) cv. Lastochka

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