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Sizes of Bacterial Cells in Soils Determined by Cascade Filtration Technique

机译:级联过滤技术测定土壤中细菌细胞的大小

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This paper studies the number of bacteria in typical chernozem and mountain-meadow soil by the traditional method and the cascade filtration technique. The total number of bacteria in these soils, which was obtained in filters of different diameters during filtering the suspension of a certain amount, is 1.5-5 times higher than that obtained by the traditional method. In the structure of the bacterial biomass in both soils, the biomass of bacterial cells with a diameter of 0.38-0.43 μm was dominating by 8-90%. In the typical chernozem, the biomass of cells with a diameter of 0.17 urn was slightly more than 1 %; in the mountain-meadow soil, the percentage of the biomass of cells with a diameter of 0.17 urn increased by 5%. The average volume and diameter of the bacteria in the studied soils were calculated. In typical chernozem, the average volume of bacterial cells was equal to 0.0046 μrn~3 and the diameter was 0.206 urn. In the mountain-meadow soils, these values were slightly lower, 0.0038 μm~3 and 0.194 μm, respectively. The biomass of the bacterial cells, which is usually calculated based on the cell volume of 0.1 μm~3, is overestimated by about five times when counting the number on the filters. The percentage of the real biomass of soil bacteria is traditionally much lower than that estimated.
机译:本文采用传统方法和级联过滤技术研究了典型黑钙土和山草甸土壤中的细菌数量。这些土壤中的细菌总数是通过一定直径的悬浮液过滤过程中在不同直径的过滤器中获得的,比传统方法获得的细菌总数高1.5-5倍。在两种土壤的细菌生物量结构中,直径为0.38-0.43μm的细菌细胞的生物量占8-90%。在典型的黑钙石中,直径为0.17微米的细胞的生物量略大于1%。在山草甸土壤中,直径为0.17 n的细胞的生物量百分比增加了5%。计算了研究土壤中细菌的平均体积和直径。在典型的黑钙土中,细菌细胞的平均体积等于0.0046μm〜3,直径为0.206 um。在山草甸土壤中,这些值略低,分别为0.0038μm〜3和0.194μm。细菌细胞的生物量通常是基于0.1μm〜3的细胞体积计算的,在过滤器上计数时,其生物量被高估了大约五倍。传统上,土壤细菌的真实生物量百分比远低于估计值。

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  • 来源
    《Biology Bulletin》 |2013年第2期|130-137|共8页
  • 作者单位

    Moscow State University, Department of Soil Science, Moscow, 119991 Russia;

    Moscow State University, Department of Soil Science, Moscow, 119991 Russia;

    Moscow State University, Department of Soil Science, Moscow, 119991 Russia;

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