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首页> 外文期刊>Biology Bulletin >Biochemical and Physiological Estimation of Barley Regenerants Obtained in Selective Systems
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Biochemical and Physiological Estimation of Barley Regenerants Obtained in Selective Systems

机译：在选择系统中获得的大麦再生体的生化和生理估计

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摘要

Callus lines of barley (Hordeum vulgare L.) resistant to aluminum toxicity and osmotic stress from which plants regenerated were obtained in selective systems. Comparative estimation of the intensity of lipid peroxidation, the content of anthocyans and ascorbic acid in leaves, the parameters of CO2/H2O gas exchange in leaves of regenerant plants obtained in selective mediums with gradual addition of aluminum ions and polyethylene glycol and in mediums without selective agents was performed in conditions of an artificial climate. The obtained data were compared with biochemical and physiological parameters of barley plants of the initial genotypes grown in the same conditions. Significant changes in the intensity of apparent photosynthesis, transpiration, and efficacy of water use by barley leaves were revealed as a result of callus culturing and especially due to the use of selective systems in vitro.
机译：在选择系统中获得了抗铝毒性和渗透胁迫的大麦（Hordeum vulgare L.）的愈伤组织系，从中再生出植物。在逐步添加铝离子和聚乙二醇的选择性培养基中以及在没有选择性培养基的条件下获得的再生植物叶片中脂质过氧化强度，花色苷和抗坏血酸含量，叶片中CO2 / H2O气体交换参数的比较估计试剂是在人工气候条件下进行的。将获得的数据与在相同条件下生长的初始基因型大麦植物的生化和生理参数进行比较。由于愈伤组织的培养，特别是由于体外选择系统的使用，显示出大麦叶片的光合作用，蒸腾作用和水分利用效率的显着变化。

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  • 来源
    《Biology Bulletin》 |2011年第6期|p.602-607|共6页
  • 作者

    I. G. Shirokikh; S. Yu. Ogorodnikova; I. V. Dalke; O. N. Shupletsova;

  • 作者单位

    Rudnitskii Zonal Northeast Research Center for Agriculture, Russian Agricultural Academy,ul. Lenina 166a, Kirov, 6/0007 Russia,Institute of Biology, Komi Research Center, Ural Branch, Russian Academy of Sciences,ul. Kommunisticheskaya 28, Syktyvkar, 167982 Russia;

    Institute of Biology, Komi Research Center, Ural Branch, Russian Academy of Sciences,ul. Kommunisticheskaya 28, Syktyvkar, 167982 Russia;

    Institute of Biology, Komi Research Center, Ural Branch, Russian Academy of Sciences,ul. Kommunisticheskaya 28, Syktyvkar, 167982 Russia;

    Rudnitskii Zonal Northeast Research Center for Agriculture, Russian Agricultural Academy,ul. Lenina 166a, Kirov, 6/0007 Russia;

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