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Disturbed Structure and Function of Chloroplasts during Blocked Biosynthesis of 5-Aminolevulinic Acid in the Light

机译:光中5-氨基乙酰丙酸的生物合成受阻期间叶绿体的结构和功能紊乱

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摘要

Xantha-702 mutant of cotton (Gossypium hirsutum L.) proved to have blocked synthesis of 5-ami-nolevulinic acid in the light. Accordingly, mutant leaves accumulated 2-5% chlorophyll of baseline. Mutant plants demonstrated disturbed production of pigment-protein complexes of photosystems Ⅰ (PSⅠ) and Ⅱ (PSⅡ) and generation of the chloroplast membrane system blocked at the early stages, largely, at the stages of vesicles and single short thylakoid. The functional activity of the PSⅠ and PSⅡ reaction centers was close to zero. Only the chlorophyll a/b light-harvesting complexes of PSⅠ and PSⅡ with the chlorophyll fluorescence peaks at 728 and 681 nm, respectively, were produced in the xantha-702 mutant. We propose that the genetic block of 5-aminolevunilic acid biosynthesis in the light in the xantha-702 mutant disturbs the formation and activity of the complexes of the reaction centers of PS-Ⅰ and PS-Ⅱ and inhibits the development of the whole membrane system of chloroplasts.
机译:事实证明,棉花的Xantha-702突变体(棉(Gossypium hirsutum L.)已阻止了5-ami-nolevulinic acid的合成。因此,突变叶累积了2-5%的叶绿素基线。突变植物表现出受干扰的光系统Ⅰ(PSⅠ)和Ⅱ(PSⅡ)的色素-蛋白质复合物的产生以及叶绿体膜系统的生成在早期(大部分在囊泡和单个短类囊体的阶段)受阻。 PSⅠ和PSⅡ反应中心的功能活性接近于零。在xantha-702突变体中,仅产生PSⅠ和PSⅡ的叶绿素a / b光捕获复合物,其叶绿素荧光峰分别在728和681 nm处。我们认为xantha-702突变体中的5-氨基乙酰丙酸生物合成的遗传阻滞干扰了PS-Ⅰ和PS-Ⅱ反应中心复合物的形成和活性,并抑制了整个膜系统的发育。叶绿体。

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  • 来源
    《Biology Bulletin》 |2007年第3期|p.248-258|共11页
  • 作者

    V. G. Ladygin;

  • 作者单位

    Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290 Russia;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物科学;
  • 关键词

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