首页> 外文期刊>Biology Bulletin >Distribution and Origin of Two Forms of the Marsh Frog Pelophylax ridibundus Complex (Anura, Ranidae) from Kamchatka Based on Mitochondrial and Nuclear DNA Data
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Distribution and Origin of Two Forms of the Marsh Frog Pelophylax ridibundus Complex (Anura, Ranidae) from Kamchatka Based on Mitochondrial and Nuclear DNA Data

机译:基于线粒体和核DNA数据的堪察加半岛两种形式的沼泽青蛙Pelophylax ridibundus复合体(阿努拉,科)的分布和起源

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The formation of the first populations of the marsh frog (the Pelophylax ridibundus complex) near Petropavlovsk-Kamchatsky and in the Paratunka River valley must have been the result of human introduction in the late 1980s. At present, more than 20 localities of this species are recorded in Kamchatka. For a more precise definition of the taxonomic status of P. ridibundus sensu lato, samples from five populations (altogether, 30 individuals) from southeastern and central Kamchatka are analyzed using molecular methods. In all frogs, a mitochondrial DNA type specific for the eastern form (=the Anatolian P. cf. bedriagae), but not for the western form (=the Central European P. ridibundus), is revealed. However, the results of nuclear DNA analysis of marsh frogs from Kamchatka reveal alleles specific for both of the forms, eastern and western, with a frequency ratio of about 2 : 1. The results of sequencing the mitochondrial ND2 gene and nuclear SAI-1 gene suggest that the ancestor individuals might have been introduced into Kamchatka from the Volga-Don interfluve or Ciscaucasia. The absence of both haplotype and nucleotide diversity in the samples studied suggests a single successful introduction that involved a low number of frogs stemming from a single locality.
机译:Petropavlovsk-Kamchatsky附近和Paratunka流域附近的沼泽蛙(Pelophylax ridibundus复合体)的第一批种群一定是人类在1980年代后期引入的结果。目前,堪察加半岛记录了该物种的20多个地方。为了更精确地定义rid.undibundus sensu lato的分类学地位,使用分子方法分析了东南部和堪察加半岛中部五个种群(总共30个个体)的样本。在所有青蛙中,都发现了特定于东部形式(= Anatolian P. cf. bedriagae)但不针对西部形式(= Central European ridibundus)的线粒体DNA类型。但是,堪察加半岛沼泽蛙的核DNA分析结果显示了两种形式(东西方)特有的等位基因,频率比约为2:1。线粒体ND2基因和SAI-1核基因测序的结果这表明祖先个体可能是从伏尔加河-顿河间流域或奇索卡西亚岛引入堪察加半岛的。在所研究的样品中既没有单倍型也没有核苷酸多样性,这表明一次成功的引入,涉及到来自一个地方的少量青蛙。

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