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首页> 外文期刊>Biology Bulletin >Isolation and Identification of Bacteria from an Ice Wedge of the Mamontova Gora Glacial Complex (Central Yakutia)
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Isolation and Identification of Bacteria from an Ice Wedge of the Mamontova Gora Glacial Complex (Central Yakutia)

机译：Mamontova Gora Gora冰川复合体的冰楔分离和鉴定（中央雅库特）

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摘要

Analysis of the aerobic heterotrophic component of bacterial communities from samples of poorly studied ancient ice wedge from the Mamontova Gora section (Central Yakutia), was carried out. The abundance of colony forming cells (CFU) of aerobic heterotrophic bacteria detected by plating of the thawed samples onto an agar nutrient media varied within 10(3)10(4) CFU/mL. At the same time, the total number of intact cells was only 1-2 orders of magnitude higher. The taxonomic position of bacteria was determined by analysis of the 16S rRNA gene sequences. These bacteria were represented by three phyla: Actinobacteria, Firmicutes, and Proteobacteria. The temperature growth range of 8-20 degrees C, which is characteristic of psychrotolerant bacteria, proved to be a specific physiological feature of the isolated strains. Most bacteria were found to be sensitive to a wide range of antibiotics.
机译：采用Mamontova Gora部分（中央雅库特）的古代冰楔的样本的细菌社区有氧异质组分的分析。通过将解冻的样品电镀到10（3）10（4）CFU / mL内的琼脂营养培养基中检测到通过电镀到琼脂营养培养基中检测到的有氧异养细菌的菌落形成细胞（CFU）。同时，完整细胞的总数仅为1-2个数量级。通过分析16S rRNA基因序列来确定细菌的分类物理位置。这些细菌由三个脑部：肌动菌，静脉曲菌和植物产生。 8-20℃的温度生长范围，其是心理侵药细菌的特征，被证明是分离菌株的特异性生理特征。发现大多数细菌对广泛的抗生素敏感。

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  • 来源
    《Biology Bulletin》 |2019年第3期|234-241|共8页
  • 作者

    Filippova S. N.; Surgucheva N. A.; Kolganova T. V.; Cherbunina M. Yu.; Brushkov A. V.; Mulyukin A. L.; Galchenko V. F.;

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    Russian Acad Sci Winogradsky Inst Microbiol Biotechnol Res Ctr Moscow 119071 Russia;

    Russian Acad Sci Winogradsky Inst Microbiol Biotechnol Res Ctr Moscow 119071 Russia;

    Russian Acad Sci Res Ctr Biotechnol Insti Bioengn Moscow 119071 Russia;

    Moscow MV Lomonosov State Univ Dept Geol Moscow 119991 Russia;

    Moscow MV Lomonosov State Univ Dept Geol Moscow 119991 Russia;

    Russian Acad Sci Winogradsky Inst Microbiol Biotechnol Res Ctr Moscow 119071 Russia;

    Russian Acad Sci Winogradsky Inst Microbiol Biotechnol Res Ctr Moscow 119071 Russia;

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