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首页> 外文期刊>Biology Bulletin >Expression of Curculin, a New Type of Alternative Sweetener in Transgenic Rice
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Expression of Curculin, a New Type of Alternative Sweetener in Transgenic Rice

机译：姜黄素的表达，转基因水稻中的一种新型替代品

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摘要

Sweet tasting and taste modifying proteins are the natural alternative to flavor enhancers and artificial sweeteners. Curculin is a unique protein, which has both sweet tasting and taste modifying properties. In this study, pinII Ti plasmid vector was constructed with curculin gene, where the gene encoding curculin protein was placed under the control of the duel cauliflower mosaic virus 35S (2x CaMV 35S) promoter into rice (Oryza sativa L. var. Japonica cv. Dongjinbyeo) by Agrobacterium-mediated transformation to generate transgenic plants. Here, twenty-six plant lines were regenerated and the transgenic rice plant lines were confirmed by different molecular analysis. The genomic PCR result revealed that all of the plant lines were transgenic. The single copied and intergenic plant lines were selected by Taqman PCR analysis and FST analysis, respectively. Expression of curculin gene in transgenic rice resulted in the accumulation of curculin protein in the leave. The transgenic lines obtained in next generation, where curculin protein was also accumulated in the plant leave. Sensory analysis result suggested that the curculin protein expressing transgenic lines exerted both sweet and taste modifying activities. These results demonstrated that curculin was expressed in transgenic rice plants.
机译：甜味品尝和味道改性蛋白质是风味增强剂和人造甜味剂的自然替代品。姜黄素是一种独特的蛋白质，具有甜味品尝和味道改性性能。在该研究中，用姜黄素基因构建Pinii Ti质粒载体，其中将编码姜黄素蛋白的基因置于Duel Cauliflower马赛克病毒35s（2x Camv 35s）启动子进入水稻（Oryza sativa L.var.paponica cv。 Dongjinbyeo）通过农杆菌介导的转化产生转基因植物。这里，再生26种植物线，通过不同的分子分析确认转基因水稻植物系。基因组PCR结果表明，所有植物线都是转基因的。通过Taqman PCR分析和FST分析选择单一复制的和非基础植物系。转基因水稻中姜黄素基因的表达导致姜黄素蛋白在休假中的积累。在下一代中获得的转基因线，其中姜黄素蛋白也积累在植物假中。感官分析结果表明，表达转基因素的姜黄素蛋白施加甜味和味道改性活性。这些结果表明，姜黄蛋白在转基因水稻植物中表达。

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