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首页> 外文期刊>Biology Bulletin >A Study of Mitochondrial DNA Copy Number and Heteroplasmy in Different Rat Brain Regions after Cranial Proton Impact
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A Study of Mitochondrial DNA Copy Number and Heteroplasmy in Different Rat Brain Regions after Cranial Proton Impact

机译：大鼠质子撞击后不同大鼠脑区线粒体DNA拷贝数和异质的研究

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Studies, accumulated over recent years, indicate that mitochondria are, along with the nucleus, the most important target of radiation damage. The structural and functional disturbances induced by radiation in these organelles influence post-radiation development in a whole complex of effects at the cellular and whole organism level in animals and humans. This study is aimed at determining changes in the number of mitochondrial DNA (mtDNA) copies relative to nuclear DNA (nDNA) and identifying mutant copies of mtDNA in three brain regions (in the hippocampus, cortex, and cerebellum) of rats, at different times after cranial proton impact. Real-time PCR and a method of cleavage of mtDNA PCR amplicon heteroduplexes with Surveyor nuclease were used in this study. We revealed that after the proton exposure, the level of the mtDNA copy content in three brain regions of rats drastically increased with a simultaneous increase in the percentage of mutant mtDNA copies. The results obtained indicate that mtDNA synthesis and the level of its mutant copies differ in the hippocampus, cortex, and cerebellum of rats after cranial exposure to protons. One can suggest that increased mtDNA mutagenesis may result in mitochondrial dysfunction with oxidative stress induction, leading to nuclear genome instability and the development of delayed effects of ionizing radiation.
机译：近年来积累的研究表明线粒体与核，辐射损伤最重要的目标。这些细胞器中辐射诱导的结构和功能性干扰在动物和人类中的细胞和整个生物水平的整个效果中影响辐射后发育。该研究旨在确定相对于核DNA（NDNA）的线粒体DNA（MTDNA）拷贝数的变化，并在不同时间鉴定三种脑区中的MTDNA突变拷贝，在不同时间在不同时间内进行大鼠的脑区（在海马，皮质，皮层和小脑中）颅骨质子撞击后。本研究使用了实时PCR和MTDNA PCR扩增子异渗患者的MTDNA PCR扩增子异渗过水性。我们透露，在质子暴露后，大鼠三个脑区中的MTDNA拷贝含量的水平随着突变体MTDNA拷贝的百分比同时增加而增加。得到的结果表明，MTDNA合成和其突变拷贝的水平在大鼠暴露于质子后大鼠的海马，皮质和大鼠的大鼠。人们可以提出增加的MTDNA诱变可能导致氧化应激诱导的线粒体功能障碍，导致核基因组不稳定性和电离辐射的延迟效应的发展。

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