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首页> 外文期刊>Biology Bulletin >Screening of Macrofungi Isolates for Aflatoxin B_1 and Ochratoxin A Degradation
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Screening of Macrofungi Isolates for Aflatoxin B_1 and Ochratoxin A Degradation

机译：用于黄曲霉毒素B_1和OCHRATOXIN的Macrofungi分离物的筛选降解

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摘要

In the present study, ninety-four macro fungi isolates were screened for aflatoxin B-1 and ochratoxin A degradation potential by their culture filtrates. Decreasing toxin amounts were determined by a semi-quantitative HPTLC method. The degradation capacity of the selected ten macrofungi isolates for each toxin was confirmed additionally by High Performance Liquid Chromatography (HPLC). The most successful isolates were determined as Lentinus strigosus OBCC 5004 and Agaricus campestris OBCC 5048 for aflatoxin B-1 and ochratoxin A degradation, respectively. Both of these species were reported as a first time for their mycotoxin degradation. The determined aflatoxin B-1 degradation rates of Lentinus strigosus with HPTLC and HPLC were 66.70 and 67.00%, respectively. On the other hand, the ochratoxin A degradation rates of Agaricus campestris were recorded as 63.20 and 56%, for both methods, respectively. Toxicity evaluation of non-degraded and degraded aflatoxin B-1 and ochratoxin A was performed by using the brine shrimp (Artemia salina) lethality assay, where 20.00 and 60.00% detoxification rates were observed for degraded AFB(1) and OTA.
机译：在本研究中，94宏真菌菌株中筛选黄曲霉毒素B-1和赭曲霉毒素可以通过培养滤液甲降解潜能。降低毒素量通过半定量HPTLC法测定。所选择的10株大型真菌每个毒素的降解容量通过高效液相色谱法（HPLC）证实附加。最成功的分离株确定为香菇strigosus OBCC 5004和分别四孢蘑菇OBCC 5048黄曲霉毒素B-1和赭曲霉毒素A降解。这两个物种中都报告为第一时间为他们的霉菌毒素的降解。香菇strigosus用HPTLC和HPLC所确定的黄曲霉毒素B-1降解率分别为66.70和67.00％之间。在另一方面，四孢蘑菇的赭曲霉毒素A降解率，分别记录为63.20和56％，对于这两种方法。通过使用其中观察到20.00和60.00％的解毒率退化AFB（1）和OTA盐水虾（卤虫）致死率试验，执行非恶化和退化黄曲霉毒素B-1 A和赭曲霉素毒性评价。

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