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首页> 外文期刊>Biologia Plantarum >Callus induction and plant regeneration from immature embryos of Brachypodium distachyon with different chromosome numbers
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Callus induction and plant regeneration from immature embryos of Brachypodium distachyon with different chromosome numbers

机译：不同染色体数短枝曲霉幼胚的愈伤组织诱导和植株再生

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The paper reports the in vitro cultivation of two commercial lines and 23 wild populations (with 10, 20 and 30 chromosomes) of Brachypodium distachyon. Callus induction was assayed on Murashige and Skoog medium containing 1 mg dm−3 2,4-dichlorophenoxyacetic acid (2,4-D) with 30 g dm−3 of sucrose (MSs) or maltose (MSm). No significant differences were seen between the two media with respect to callus induction. Calli were transferred to MSm medium without 2,4-D but containing 0.1 mg dm−3 of 6-benzylaminopurine for plant regeneration. The plant regeneration response was very variable depending on the original induction medium, although no overall preference for one or the other medium was seen. The three main culture stages (callus induction, plant regeneration, and green plantlets formation) are probably differently controlled in the plants with different chromosome numbers. This supports the idea that the three cytotypes of Brachypodium cultured actually belong to different species.
机译：该论文报道了短枝短螺旋藻的两个商业品系和23个野生种群（具有10、20和30个染色体）的体外培养。在含有1 mg dm -3 2,4-二氯苯氧基乙酸（2,4-D）和30 g dm -3 蔗糖的Murashige和Skoog培养基上测定愈伤组织的诱导（MSs）或麦芽糖（MSm）。在愈伤组织诱导方面，两种培养基之间未见明显差异。将愈伤组织转移至无2,4-D，但含有0.1 mg dm -3 的6-苄基氨基嘌呤的MSm培养基中进行植物再生。植物再生响应取决于原始的诱导培养基是非常可变的，尽管没有看到对一种或另一种培养基的总体偏好。在具有不同染色体数目的植物中，三个主要培养阶段（愈伤组织诱导，植物再生和绿色小植株形成）的控制方式可能不同。这支持了这样的想法，即培养的腕足动物的三种细胞类型实际上属于不同的物种。

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