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首页> 外文期刊>Biomass & bioenergy >Metagenomic analysis of autochthonous microbial biomass from banana waste: Screening design of factors that affect hydrogen production
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Metagenomic analysis of autochthonous microbial biomass from banana waste: Screening design of factors that affect hydrogen production

机译：香蕉垃圾自加湿微生物生物量分析：筛选氢气产量的筛选设计

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摘要

In this study, Plackett-Burman designs were applied to evaluate the effects of independent variables (pH 5.5-7.7, temperature 30-44 °C, headspace volume 40-60%, percentage of inoculum 5-15% and carbohydrates concentration 3-15 g L~(-1)) on fermentative hydrogen production by autochthonous bacteria from banana waste. The screening experiments showed only pH, temperature and headspace were significant variables affecting hydrogen production (PH_2), in which the highest PH_2 of 38.08 mL was obtained at pH 7.5, 44 °C, 40% headspace, 15% inoculum and 15 g L~(-1) carbohydrates concentration. Clostridium and Lactobacillus were the most abundant autochthonous acidogenic bacteria and were associated with the production of acetic acid (0.02-0.72 g L~(-1)), butyric acid (0.03-2.66 g L~(-1)), lactic acid (0.26-5.14 g L~(-1)) and ethanol (0.08-0.64 g L~(-1)) in the assays. Genes related to carbohydrate metabolism, acidogenesis and H_2 production enzymes were identified, such as fructokinase, glucose 6-phosphate dehydrogenase, lactate dehydrogenase and pyruvate ferredoxin oxidoreductase.
机译：在本研究中，应用Plackett-Burman设计来评估自变量的影响（pH5-7.7，温度30-44°C，前空体积40-60％，接种物的百分比5-15％，碳水化合物浓度3-15香蕉垃圾上的自身加热细菌产生的Gl〜（-1））。筛选实验仅显示pH，温度和顶部空间是影响氢气产生（pH_2）的显着变量，其中在pH 7.5,44℃，40％的顶部空间，15​​％接种物和15g L〜中获得38.08ml的最高pH_2。 （-1）碳水化合物浓度。 Clostridium和乳杆菌是最丰富的酸性酸性细菌，与乙酸的产生有关（0.02-0.72g l〜（-1）），丁酸（0.03-2.66g l〜（-1）），乳酸（测定中0.26-5.14g L〜（-1））和乙醇（0.08-0.64g l〜（-1））。鉴定了与碳水化合物代谢，酸性生成和H_2生产酶相关的基因，例如果糖酶，葡萄糖6-磷酸脱氢酶，乳酸脱氢酶和丙酮酸芳米氧化酶氧化还原酶。

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  • 来源
    《Biomass & bioenergy》 |2020年第7期|105573.1-105573.11|共11页
  • 作者

    Raissa Cristina da Silva Mazareli; Alejandra Carolina Villa-Montoya; Tiago Palladino Delforno; Victor Borin Centurion; Valeria Maia de Oliveira; Edson Luiz Silva; Maria Bernadete Amancio Varesche;

  • 作者单位

    Laboratory of Biological Processes Department of Hydraulics and Sanitation Sao Carlos School of Engineering University of Sao Paulo Campus Ⅱ Sao Carlos SP CEP 13563-120 Brazil;

    Laboratory of Biological Processes Department of Hydraulics and Sanitation Sao Carlos School of Engineering University of Sao Paulo Campus Ⅱ Sao Carlos SP CEP 13563-120 Brazil;

    Microbial Resources Division Research Center for Chemistry Biology and Agriculture (CPQBA) Campinas University Campinas SP CEP 13081-970 Brazil;

    Microbial Resources Division Research Center for Chemistry Biology and Agriculture (CPQBA) Campinas University Campinas SP CEP 13081-970 Brazil;

    Microbial Resources Division Research Center for Chemistry Biology and Agriculture (CPQBA) Campinas University Campinas SP CEP 13081-970 Brazil;

    Center of Exact Sciences and Technology Department of Chemical Engineering Federal University of Sao Carlos Sao Carlos SP CEP 13565-905 Brazil;

    Laboratory of Biological Processes Department of Hydraulics and Sanitation Sao Carlos School of Engineering University of Sao Paulo Campus Ⅱ Sao Carlos SP CEP 13563-120 Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Microbial communities; Bioinformatics; Renewable energy; Organic acids; Plackett-Burman design;

    机译：微生物社区;生物信息学;再生能源;有机酸;Plackett-Burman设计;

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