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首页> 外文期刊>Biomass & bioenergy >Direct bioconversion of sorghum extract sugars to free fatty acids using metabolically engineered Escherichia coli strains: Value addition to the sorghum bioenergy crop
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Direct bioconversion of sorghum extract sugars to free fatty acids using metabolically engineered Escherichia coli strains: Value addition to the sorghum bioenergy crop

机译：使用代谢工程大肠杆菌菌株将高粱提取糖直接生物转化为游离脂肪酸：高粱生物能源作物的增值

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摘要

The unprecedented challenge of meeting the energy demand of growing economies has increased the need to produce hydrocarbon fuels using renewable sources. Among different platforms being researched these days, hydrocarbon biofuel and chemical production from free fatty acids (FFAs) have gained a lot of attention. The current study reports on value addition to sorghum energy crop through utilization of sorghum extract as a renewable carbon source for FFA production using genetically engineered Escherichia coli. In order to reduce the production cost of FFAs, direct sucrose utilizing £ coli strains were developed. The E. coli strain MG1655 with fadD mutant (named as ML103) either carrying the plasmid bearing the gene for acyl-ACP thioesterase (TE) from Ricinus communis (pXZ18) or a plasmid bearing a combination of the TE and the native (3R)-hydroxyacyl-ACP dehydrase gene (fabZ) (pXZ18Z) was modified to utilize sucrose by incorporating the pUR400 plasmid. The newly created strains utilized a mixture of pure sugars and sorghum extract efficiently. The 24 h pH adjusted culture of ML103 pXZ18Z pUR400 produced a maximum of 6.18 ± 0.52 g l~(-1) from 30 g l~(-1) of pure sugar mixture and 2.95 ± 0.04 g l~(-1) with sorghum extract (15 g l~(-1) equivalent sugar concentration). These data suggest that modified £ coli strains were capable of directly utilizing sucrose and produce FFAs from it. Successful demonstration of direct bioconversion of sorghum extract to FFA was validated as a possible value addition of the sorghum bioenergy crop.
机译：满足增长中经济体能源需求的空前挑战，增加了使用可再生资源生产碳氢燃料的需求。在当今正在研究的不同平台中，烃类生物燃料和游离脂肪酸（FFA）的化学生产引起了广泛关注。当前的研究报道了通过利用高粱提取物作为转基因碳，利用转基因大肠杆菌生产高FFA来增加高粱能源作物的价值。为了降低FFA的生产成本，开发了利用大肠杆菌菌株的直接蔗糖。带有fadD突变体（命名为ML10​​3）的大肠杆菌MG1655菌株，带有携带来自蓖麻的酰基ACP硫酯酶（TE）基因的质粒（pXZ18）或带有TE与天然氨基酸（3R）结合的质粒通过掺入pUR400质粒，修饰-羟酰基-ACP脱水酶基因（fabZ）（pXZ18Z）以利用蔗糖。新产生的菌株有效地利用了纯糖和高粱提取物的混合物。 ML103 pXZ18Z pUR400的24 h pH调节培养物从30 gl〜（-1）纯糖混合物和2.95±0.04 gl〜（-1）用高粱提取物产生的最大产量为6.18±0.52 gl〜（-1）（15 gl〜（-1）当量糖浓度）。这些数据表明，改良的大肠杆菌菌株能够直接利用蔗糖并从中产生FFA。高粱提取物直接生物转化为FFA的成功示范已被证实是高粱生物能源作物的可能增值。

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  • 来源
    《Biomass & bioenergy》 |2016年第10期|217-226|共10页
  • 作者

    Mahesh Bule; Yane Luo; George Bennett; Mukund Karanjikar; William Rooney; Ka-Yiu San;

  • 作者单位

    Department of Bioengineering, Rice University, Houston, TX, USA,Technology Holding LLC, Salt Lake City, UT 84103, USA;

    Shaanxi Key Laboratory of Biomedical Materials, Northwest University, Xi'an, China;

    Department of Biochemistry and Cell Biology, Rice University, Houston, TX, USA;

    Technology Holding LLC, Salt Lake City, UT, USA;

    Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA;

    Department of Bioengineering, Rice University, Houston, TX 77030, USA,Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Free fatty acid; Escherichia coli; Sorghum extract; Hydrocarbon fuel; Value addition;

    机译：游离脂肪酸;大肠杆菌;高粱提取物;碳氢燃料;增值;

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