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首页> 外文期刊>Biomass & bioenergy >Pretreatment of Japanese cedar wood by white rot fungi and ethanolysis for bioethanol production
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Pretreatment of Japanese cedar wood by white rot fungi and ethanolysis for bioethanol production

机译：白腐真菌和乙醇分解法预处理日本柳杉木材用于生产生物乙醇

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摘要

Japanese cedar (Cryptomeria japonica) shares around 60% of plantation forests in Japan, and there is a growing demand for thinning of the forest. However, the softwood is one of the most recalcitrant wood species for hydrothermal and thermochemical pretreatments for enzymatic saccharification. In the present paper, we applied combined pretreatments by solvolysis and cultivation with white rot fungi to develop environmentally benign pretreatment system applicable to recalcitrant softwood. Due to the recalcitrance of the softwood, enzymatic saccharification yield from ethanolysis pulp was 10.2%, based on the weight of holocellulose. To increase the sugar yield, the softwood was treated with selective white rot fungi prior to the ethanolysis. Treatment of the softwood with a bio- pulping fungus, Ceriporiopsis subuermispora FP-90031 and a new fungal isolate Phellinus sp. SKM2102 for 8 weeks increased the sugar yield to 35.7 and 40.8%, respectively. The best pretreatment conditions in terms of overall sugar yield including a soluble fraction were obtained by ethanolysis after the fugal treatment with Phellinus sp. SKM2102, resulting in production of 42.2 g of total reducing sugars per 100 g of the fungus-pretreated biomass. After the combined pretreatment, simultaneous saccharification and fermentation of the water-insoluble pulp fraction were carried out using Saccharomyces cereuisiae. Ethanol production from undecayed Japanese cedar wood was negligible but pretreatments with the two fungi significantly increased the ethanol production, in combination with ethanolysis. The combined pretreatment with solvolysis and Phellinus sp. SKM2102 is attractive for biorefinery of the recalcitrant softwood.
机译：日本柳杉（Cryptomeria japonica）在日本的人工林中占有约60％的份额，对森林砍伐的需求也越来越大。然而，软木是用于酶促糖化的水热和热化学预处理的最顽固的木材种类之一。在本文中，我们将溶剂分解和白腐真菌栽培相结合的预处理方法应用于开发适用于顽固软木的环境友好的预处理系统。由于软木的顽固性，基于全纤维素的重量，乙醇化纸浆的酶促糖化产率为10.2％。为了增加糖产量，在乙醇分解之前，用选择性的白腐真菌处理软木。用生物制浆真菌，Ceriporiopsis subuermispora FP-90031和新的真菌分离菌Phellinus sp处理软木。连续8周，SKM2102的糖产量分别提高到35.7和40.8％。在用桑黄菌进行真菌处理后，通过乙醇解法获得了包括可溶部分在内的总糖产量方面的最佳预处理条件。 SKM2102，每100克经真菌预处理的生物量可产生42.2克总还原糖。组合的预处理之后，使用酿酒酵母（Saccharomyces cereuisiae）同时进行糖化和水不溶性果肉级分的发酵。来自未腐烂的日本柏木的乙醇产量可以忽略不计，但是与两种真菌一起预处理可以显着提高乙醇产量，同时还可以进行乙醇分解。溶剂分解和桑黄组合预处理。 SKM2102对顽固软木的生物精炼具有吸引力。

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  • 来源
    《Biomass & bioenergy》 |2011年第1期|p.320-324|共5页
  • 作者

    Yasunori Baba; Toshiaki Tanabe; Nobuaki Shirai; Takahito Watanabe; Yoichi Honda; Takashi Watanabe;

  • 作者单位

    Research Institute for Sustainable Humanosphere, Kyoto University, Gofeasho, Uji, Kyoto 611-0011, Japan;

    Research Institute for Sustainable Humanosphere, Kyoto University, Gofeasho, Uji, Kyoto 611-0011, Japan;

    Industrial Research Center o/Shiga Prefecture, Kamitoyama 232, Ritto, Shiga 520-3004, Japan;

    Research Institute for Sustainable Humanosphere, Kyoto University, Gofeasho, Uji, Kyoto 611-0011, Japan;

    Research Institute for Sustainable Humanosphere, Kyoto University, Gofeasho, Uji, Kyoto 611-0011, Japan;

    Research Institute for Sustainable Humanosphere, Kyoto University, Gofeasho, Uji, Kyoto 611-0011, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    bioethanol; enzymatic saccharification and; fermentation; pretreatment; white rot fungi; ethanolysis; organosolvolysis;

    机译：生物乙醇酶促糖化;发酵预处理白腐真菌乙醇分解有机溶剂分解;

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