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首页> 外文期刊>Biomedical Microdevices >Fabrication of two-layer poly(dimethyl siloxane) devices for hydrodynamic cell trapping and exocytosis measurement with integrated indium tin oxide microelectrodes arrays
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Fabrication of two-layer poly(dimethyl siloxane) devices for hydrodynamic cell trapping and exocytosis measurement with integrated indium tin oxide microelectrodes arrays

机译：集成铟锡氧化物微电极阵列的用于流体动力细胞捕获和胞吐测量的两层聚二甲基硅氧烷器件的制造

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摘要

The design, fabrication and test of a microfluidic cell trapping device to measure single cell exocytosis were reported. Procedures on the patterning of double layer template based on repetitive standard photolithography of AZ photoresist were investigated. The replicated poly(dimethyl siloxane) devices with 2.5 μm deep channels were proved to be efficient for stopping cells. Quantal exocytosis measurement can be achieved by targeting single or small clumps of chromaffin cells on top of the 10 μm×10μm indium tin oxide microelectrodes arrays with the developed microdevice. And about 72 % of the trapping sites can be occupied by cells with hydrodynamic trapping method and the recorded amperomet-ric signals are comparable to the results with traditional carbon fiber microelectrodes. The method of manufacturing the microdevices is simple, low-cost and easy to perform. The manufactured device offers a platform for the high throughput detection of quantal catecholamine exocytosis from chromaffin cells with sufficient sensitivity and broad application.
机译：报道了用于测量单细胞胞吐作用的微流细胞捕获装置的设计，制造和测试。研究了基于AZ光刻胶的重复标准光刻对双层模板进行构图的程序。事实证明，具有2.5μm深通道的复制的聚（二甲基硅氧烷）装置对于阻止细胞有效。可以通过将单个或小的嗜铬细胞团块靶向10微米×10微米的铟锡氧化物微电极阵列顶部来实现量子胞吐作用的测量。流体动力俘获方法可将细胞占据大约72％的俘获位点，并且所记录的安培信号可与传统碳纤维微电极的结果相媲美。制造微器件的方法简单，低成本且易于执行。所制造的设备提供了一个平台，可以以足够的灵敏度和广泛的应用来从嗜铬细胞中高通量检测儿茶酚胺定量胞吐作用。

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  • 来源
    《Biomedical Microdevices》 |2013年第3期|445-451|共7页
  • 作者

    Changlu Gao; Xiuhua Sun; Kevin D. Gillis;

  • 作者单位

    School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China;

    School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China;

    Dalton Cardiovascular Research Center, University of Missouri at Columbia, Columbia, MO 65211, USA;

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  • 正文语种 eng
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  • 关键词

    microfluidic; amperometric; quantal exocytosis;

    机译：微流体安培定量胞吐;

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