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首页> 外文期刊>Biomedical Microdevices >Spontaneous formation of stably-attached and 3D-organized hepatocyte aggregates on oxygen-permeable polydimethylsiloxane membranes having 3D microstructures
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Spontaneous formation of stably-attached and 3D-organized hepatocyte aggregates on oxygen-permeable polydimethylsiloxane membranes having 3D microstructures

机译：在具有3D微观结构的透氧性聚二甲基硅氧烷膜上自发形成稳定附着的3D组织的肝细胞聚集体

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摘要

In order to enhance the viability and the differentiated functions of primary hepatocytes in cultures, it appears important to have them organized within a three-dimensional (3D) structure which promotes extensive cell-cell contacts, but also to be adequately supplied with oxygen. Here, we report a simple methodology satisfying these two fundamental but sometimes conflicting issues: primary rat hepatocytes were cultured on polydimethylsiloxane (PDMS) membranes with 3D-pillared microstructures with various dimensions, so that the cells could organize themselves around the pillars into various kinds of 3D multicellular aggregates, while being continuously supplied with oxygen by diffusion through the PDMS membrane. As expected, under such conditions, hepatocyte cultures exhibited higher albumin secretion and urea synthesis rates. It appeared then that as the spacing decreased between the pillars, the cells were more stably organized into smaller spherical aggregates and displayed the highest albumin secretion rates. Such a simple design is likely to be included in a new drug/chemical screening system in a practical microplate format, but also appears applicable to microfluidic devices.
机译：为了提高培养物中原代肝细胞的活力和分化功能，将它们组织在一个三维（3D）结构中很重要，该结构可促进广泛的细胞与细胞之间的接触，但也要有充足的氧气。在这里，我们报告了一种简单的方法，可以满足这两个基本但有时是相互矛盾的问题：将大鼠原代肝细胞培养在具有各种尺寸的3D柱状微结构的聚二甲基硅氧烷（PDMS）膜上，以便细胞可以围绕支柱组织成各种类型的3D多细胞聚集体，同时通过扩散穿过PDMS膜而连续提供氧气。如预期的那样，在这种条件下，肝细胞培养物表现出更高的白蛋白分泌和尿素合成速率。这样看来，随着柱子之间的间距减小，细胞更稳定地组织成较小的球形聚集体，并显示出最高的白蛋白分泌率。这种简单的设计可能会以实用的微孔板形式包含在新的药物/化学筛选系统中，但似乎也适用于微流体设备。

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  • 来源
    《Biomedical Microdevices》 |2010年第3期|465-475|共11页
  • 作者

    Fanny Evenou; Teruo Fujii; Yasuyuki Sakai;

  • 作者单位

    Laboratory for Integrated Micro-Mechatronic Systems (LIMMS/CNRS-IIS), Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan;

    rnInstitute of Industrial Science (IIS), University of Tokyo,4-6-1 Komaba,Meguro-ku, Tokyo 153-8505, Japan;

    rnInstitute of Industrial Science (IIS), University of Tokyo,4-6-1 Komaba,Meguro-ku, Tokyo 153-8505, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    rat hepatocyte; oxygen supply; PDMS membrane; 3D structure; 3D aggregate;

    机译：大鼠肝细胞氧气供应;PDMS膜;3D结构;3D聚合;

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