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首页> 外文期刊>Bioorganic and Medicinal Chemistry >New amino and acetamido monomethine cyanine dyes for the detection of DNA in agarose gels
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New amino and acetamido monomethine cyanine dyes for the detection of DNA in agarose gels

机译：用于检测琼脂糖凝胶中DNA的新型氨基和乙酰氨基单甲胺花青染料

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摘要

Some new monomethine cyanine dyes derived from quinoline and benzothiazole have been prepared and characterized by ~1H and ~(13)C NMR, FTIR, FABHRMS, and visible spectroscopy. The dyes containing amino and acetamido groups were conveniently synthesized by the condensation of two p-toluenesulfonate heterocyclic quaternary salts and were obtained in the forms of iodide, bromide, and tosylate counteranions. These dyes were compared to ethidium bromide as stains for DNA in electrophoretic gels. The overall results obtained for the sensitivity of these dyes suggest the suitability of acetamido moiety over the amine one and bromide as the counteranion when compared with iodide and tosylate, with a similar capacity of DNA detection in relation to the ethidium bromide stain over the concentration range of 1-3 ng.
机译：制备了一些新的衍生自喹啉和苯并噻唑的单次甲基花青染料，并通过〜1H和〜（13）C NMR，FTIR，FABHRMS和可见光谱进行了表征。通过两种对甲苯磺酸盐杂环季盐的缩合，可以方便地合成含有氨基和乙酰氨基的染料，并以碘化物，溴化物和甲苯磺酸根抗衡阴离子的形式获得。将这些染料与溴化乙锭作比较，作为电泳凝胶中DNA的染色剂。对于这些染料的敏感性获得的总体结果表明，与碘化物和甲苯磺酸盐相比，乙酰胺基部分对胺一和溴化物作为抗衡阴离子的适用性，在浓度范围内与溴化乙锭染色相关的DNA检测能力相似。 1-3 ng。

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