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首页> 外文期刊>Bioprocess and Biosystems Engineering >Influence of culture conditions towards optimal carotenoid production by Gordonia alkanivorans strain 1B
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Influence of culture conditions towards optimal carotenoid production by Gordonia alkanivorans strain 1B

机译：培养条件对Gordonia alkanivorans菌株1B产生最佳类胡萝卜素的影响

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摘要

With the increasing awareness on the toxicity of several synthetic dyes, demand for pigments from natural sources, such as microbial carotenoids, has gained interest as a promising safe alternative colour additive. In this study, a surface response methodology based on the Doehlert distribution for two factors [% of glucose in a mixture of glucose + fructose (10 g/L total sugars), and sulfate concentration] was used towards the optimal carotenoids production by Gordonia alkanivorans strain 1B in the presence of light (400 lx). Time influence on pigment production by this bacterium was also evaluated, as well as the cell viability profile during longer incubation periods at optimal conditions. Indeed, the highest carotenoid production (2596-3100 mu g/g(DCW)) was obtained when strain 1B was cultivated in the optimal conditions: glucose 10 g/L and sulfate >= 22 mg/L, in the presence of light for 19 days at 30 degrees C, 150 rpm. Flow cytometry showed that the highest production was somehow related with the cellular stress. These results highlight the great potential of strain 1B as a new hyperpigment producer to be exploited towards several applications.
机译：随着人们对几种合成染料毒性的认识不断提高，人们对天然来源的颜料（例如微生物类胡萝卜素）的需求日益受到关注，作为一种有前途的安全替代色料。在这项研究中，基于Doehlert分布的两个因素[葡萄糖+果糖混合物中的葡萄糖％（总糖10 g / L）和硫酸盐浓度]的表面响应方法用于Gordonia alkanivorans的最佳类胡萝卜素生产在光（400 lx）存在下的菌株1B。还评估了这种细菌对色素产生的时间影响，以及在最佳条件下较长孵育时间期间的细胞活力。的确，当在最佳条件下培养菌株1B：葡萄糖10 g / L和硫酸盐> = 22 mg / L，在有光照的条件下培养时，可获得最高的类胡萝卜素产量（2596-3100μg/ g（DCW））。在30摄氏度，150 rpm下运行19天。流式细胞仪显示最高产量与细胞应激有关。这些结果凸显了菌株1B作为一种新的色素沉着产生剂的巨大潜力，可被用于多种应用。

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