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Organic acid pretreatment of oil palm trunk: effect on enzymatic saccharification and ethanol production

机译：油棕树干的有机酸预处理：对酶促糖化和乙醇生产的影响

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Effective lignocellulosic biomass saccharification is one of the crucial requirements of biofuel production via fermentation process. Organic acid pretreatments have been gained much interests as one of the high potential methods for promoting enzymatic saccharification of lignocellulosic materials due to their lower hazardous properties and lower production of inhibitory by-products of fermentation than typical chemical pretreatment methods. In this study, three organic acids, including acetic acid, oxalic acid, and citric acid, were examined for improvement of enzymatic saccharification and bioethanol production from oil palm trunk biomass. Based on response surface methodology, oxalic acid pretreated biomass released the maximum reducing sugar of 144 mg/g-pretreated biomass at the optimum condition, which was higher than untreated samples for 2.30 times. The released sugar yield of oil palm trunk also corresponded to the results of FT-IR analysis, which revealed the physical modification of cellulose and hemicellulose surface structures of pretreated biomass. Nevertheless, citric acid pretreatment is the most efficient pretreatment method to improve bioethanol fermentation of Saccharomyces cerevisiae TISTR 5606 at 1.94 times higher than untreated biomass. These results highlighted the selection of organic acid pretreatment as a potential method for biofuel production from oil palm trunk feedstocks.
机译：有效的木质纤维素生物质糖化是通过发酵过程生产生物燃料的关键要求之一。作为促进木质纤维素材料的酶促糖化的高潜力方法之一，有机酸预处理由于与常规化学预处理方法相比具有较低的危险性和较低的发酵抑制副产物而得到了广泛的关注。在这项研究中，检查了三种有机酸，包括乙酸，草酸和柠檬酸，以改善酶促糖化作用和油棕树干生物质生产生物乙醇的能力。根据响应面方法，草酸预处理的生物质在最佳条件下释放的最大还原糖为144 mg / g预处理的生物质，比未处理的样品高2.30倍。油棕树干释放的糖产量也对应于FT-IR分析的结果，该结果揭示了预处理生物质中纤维素和半纤维素表面结构的物理修饰。然而，柠檬酸预处理是提高酿酒酵母TISTR 5606的生物乙醇发酵效率的最有效的预处理方法，其效率是未处理生物质的1.94倍。这些结果突出了选择有机酸预处理作为从油棕树干原料生产生物燃料的潜在方法。

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