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首页> 外文期刊>Bioprocess and Biosystems Engineering >Biocatalysis of aromatic benzyl-propionate ester by different immobilized lipases
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Biocatalysis of aromatic benzyl-propionate ester by different immobilized lipases

机译：固定化脂肪酶对芳族丙酸苄酯的生物催化作用

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摘要

Benzyl propionate is an aromatic ester that possesses a fruity odor and is usually found in nature in the composition of some fruits such as plums and melons. This work aimed for the benzyl propionate synthesis by esterification using a new immobilized enzyme preparation with low-cost material from Candida antarctica (NS 88011) and three commercial immobilized lipases (Novozym 435, Lipozyme TL-IM and Lipozyme RM-IM). Novozym 435 had the best performance even when the solvent tert-butanol was absent of the reaction medium. Results from a 2(2) factorial design showed that an increase in the enzyme amount led to a higher conversion, even when the temperature was kept at the low value. Currently, no research had synthesized successfully benzyl propionate via esterification mediated by lipases; and we reached an ester conversion of similar to 44% after 24 h indicating that it is a promising route for benzyl propionate biotechnological production.
机译：丙酸苄酯是一种具有果味的芳香酯，通常在自然界中的某些水果（如李子和甜瓜）的成分中发现。这项工作旨在通过使用新型固定化酶制剂和南极假丝酵母的低成本原料（NS 88011）和三种商业化固定化脂肪酶（Novozym 435，Lipozyme TL-IM和Lipozyme RM-IM）进行酯化合成丙酸苄酯。即使在反应介质中没有溶剂叔丁醇时，Novozym 435仍具有最佳性能。 2（2）析因设计的结果表明，即使温度保持在较低的水平，酶量的增加也会导致较高的转化率。目前，尚无研究通过脂肪酶介导的酯化反应成功合成丙酸苄酯。并且在24小时后，我们的酯转化率接近44％，这表明这是丙酸苄酯生物技术生产的有希望的途径。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第5期|585-591|共7页
  • 作者

    Almeida Sa Amanda Gomes; de Meneses Alessandra Cristina; Lerin Lindomar Alberto; Hermes de Araujo Pedro Henrique; Sayer Claudia; de Oliveira Debora;

  • 作者单位

    Univ Fed Santa Catarina, Lab Controle Proc, Dept Engn Quim & Engn Alimentos EQA, Campus Trindade,Cx Postal 476, BR-88010970 Florianopolis, SC, Brazil;

    Univ Fed Santa Catarina, Lab Controle Proc, Dept Engn Quim & Engn Alimentos EQA, Campus Trindade,Cx Postal 476, BR-88010970 Florianopolis, SC, Brazil;

    Univ Fed Santa Catarina, Lab Controle Proc, Dept Engn Quim & Engn Alimentos EQA, Campus Trindade,Cx Postal 476, BR-88010970 Florianopolis, SC, Brazil;

    Univ Fed Santa Catarina, Lab Controle Proc, Dept Engn Quim & Engn Alimentos EQA, Campus Trindade,Cx Postal 476, BR-88010970 Florianopolis, SC, Brazil;

    Univ Fed Santa Catarina, Lab Controle Proc, Dept Engn Quim & Engn Alimentos EQA, Campus Trindade,Cx Postal 476, BR-88010970 Florianopolis, SC, Brazil;

    Univ Fed Santa Catarina, Lab Controle Proc, Dept Engn Quim & Engn Alimentos EQA, Campus Trindade,Cx Postal 476, BR-88010970 Florianopolis, SC, Brazil;

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  • 正文语种 eng
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  • 关键词

    Flavor; Enzyme; Esterification; Synthesis; Benzyl propionate;

    机译：风味;酶;酯化;合成;丙酸苄酯;

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