...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Heterologous expression of two Aspergillus niger feruloy esterases in Trichoderma reesei for the production of ferulic acid from wheat bran
【24h】

Heterologous expression of two Aspergillus niger feruloy esterases in Trichoderma reesei for the production of ferulic acid from wheat bran

机译：两种米曲霉阿魏酸酯酶在里氏木霉中的异源表达，用于由麦麸生产阿魏酸

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Feruloyl esterase (FAE)-encoding genes AnfaeA and AnfaeB were isolated from Aspergillus niger 0913. For overexpression of the two genes in Trichoderma reesei, constitutive and inductive expression plasmids were constructed based on parental plasmid pAg1-H3. The constructed plasmids contained AnfaeA or AnfaeB gene under the control of glyceraldehyde-3-phosphate dehydrogenase A gene (gpdA) promoter (from A. nidulans) or cellobiohydrolases I (cbh I) gene promoter (from T. reesei), and cbh I terminator from T. reesei. The target plasmids were transferred into T. reesei D-86271 (Rut-C30) by Agrobacterium tumefaciens-mediated transformation (ATMT), respectively. A high level of feruloyl esterase was produced by the recombinant fungal strains under solid-state fermentation, and the cbh I promoter was more efficient than the gpdA promoter in the expression of AnfaeA. The optimum temperatures and pH values were 50 degrees C and 5.0 for AnFAEA, and 35 degrees C and 6.0 for AnFAEB. The maximum production levels were 20.69 U/gsd for AnFAEA and 15.08 U/gsd for AnFAEB. The recombinant fungal enzyme systems could release 62.9% (for AnFAEA) and 52.2% (for AnFAEB) of total ferulic acids from de-starched wheat bran, which was higher than the 46.3% releasing efficiency of A. niger 0913. The supplement of xylanase from T. longibrachiatum in the enzymatic hydrolysis led to a small increment of the ferulic acids release.
机译：从黑曲霉0913中分离出编码阿魏酸酯酶（FAE）的基因AnfaeA和AnfaeB。为了在里氏木霉中过表达两个基因，基于亲本质粒pAg1-H3构建了组成型和诱导表达质粒。所构建的质粒包含在甘油醛-3-磷酸脱氢酶A基因（gpdA）启动子（来自构巢曲霉）或纤维二糖水解酶I（cbh I）基因启动子（来自里氏木霉）的控制下的AnfaeA或AnfaeB基因，以及cbh I终止子来自里氏木霉。通过根癌农杆菌介导的转化（ATMT）分别将靶质粒转移到里氏木霉D-86271（Rut-C30）中。在固态发酵下，通过重组真菌菌株产生了高水平的阿魏酸酯酶，并且在AnfaeA的表达中，cbh I启动子比gpdA启动子更有效。最佳温度和pH值对于AnFAEA为50摄氏度和5.0，对于AnFAEB为35摄氏度和6.0。 AnFAEA的最高产量为20.69 U / gsd，AnFAEB的最高产量为15.08 U / gsd。重组真菌酶体系可从解淀粉的麦麸中释放出总阿魏酸的62.9％（AnFAEA）和52.2％（AnFAEB），高于黑曲霉0913的4​​6.3％释放效率。木聚糖酶的补充来自长臂梭菌的酶促水解导致阿魏酸释放的少量增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号