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首页> 外文期刊>Bioprocess and Biosystems Engineering >Genome shuffling and ribosome engineering of Streptomyces virginiae for improved virginiamycin production
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Genome shuffling and ribosome engineering of Streptomyces virginiae for improved virginiamycin production

机译：弗吉尼亚链霉菌的基因组改组和核糖体工程可提高弗吉尼亚霉素的产量

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摘要

The production of virginiamycin (VGM) from Streptomyces virginiae was improved by genome shuffling and ribosome engineering companied with a high-throughput screening method integrating deep-well cultivation and the cylinder-plate detecting. First, a novel high-throughput method was developed to rapidly screen large numbers of VGM-producing mutants. Then, the starting population of genome shuffling was obtained through ultraviolet (UV) and microwave mutagenesis, and four mutants with higher productivity of VGM were selected for genome shuffling. Next, the parent protoplasts were inactivated by UV and heat when a fusant probability was about 98%. Streptomycin resistance was used as an evolutionary pressure to extend positive effects on VGM synthesis. Finally, after five rounds of genome shuffling, a genetically stable strain G5-103 was obtained and characterized to be able to yield 251 mg/L VGM, which was 3.1- and 11.6-fold higher than that of the mutant strain UV 1150 and the wild-type strain, respectively.
机译：通过基因组改组和核糖体工程以及结合深孔培养和圆筒板检测的高通量筛选方法，可以改善弗吉尼亚州链霉菌的弗吉尼亚霉素（VGM）的生产。首先，开发了一种新颖的高通量方法来快速筛选大量产生VGM的突变体。然后，通过紫外线（UV）和微波诱变获得了基因组改组的起始种群，并选择了四个具有更高产量的VGM突变体进行基因组改组。接下来，当融合概率约为98％时，母体原生质体会被紫外线和热灭活。链霉素抗性用作进化压力，以扩展对VGM合成的积极作用。最终，经过五轮基因组改组，获得了遗传稳定的菌株G5-103，其特征在于能够产生251 mg / L VGM，比突变菌株UV 1150和UV 1150分别高3.1倍和11.6倍。野生型菌株。

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