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首页> 外文期刊>Bioprocess and Biosystems Engineering >Improved fermentation performance to produce bioethanol from Gelidium amansii using Pichia stipitis adapted to galactose
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Improved fermentation performance to produce bioethanol from Gelidium amansii using Pichia stipitis adapted to galactose

机译：使用适应半乳糖的毕赤酵母提高发酵性能以从金盏花生产生物乙醇

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摘要

This study employed a statistical method to obtain optimal hyper thermal acid hydrolysis conditions using Gelidium amansii (red seaweed) as a source of biomass. The optimal hyper thermal acid hydrolysis using G. amansii as biomass was determined as 12% (w/v) slurry content, 358.3 mM H2SO4, and temperature of 142.6 A degrees C for 11 min. After hyper thermal acid hydrolysis, enzymatic saccharification was carried out. The total monosaccharide concentration was 45.1 g/L, 72.2% of the theoretical value of the total fermentable monosaccharides of 62.4 g/L based on 120 g dry weight/L in the G. amansii slurry. To increase ethanol production, 3.8 g/L 5-hydroxymethylfurfural (HMF) in the hydrolysate was removed by treatment with 3.5% (w/v) activated carbon for 2 min and fermented with Pichia stipitis adapted to high galactose concentrations via separate hydrolysis and fermentation. With complete HMF removal and the use of P. stipitis adapted to high galactose concentrations, 22 g/L ethanol was produced (yield 0.50). Fermentation with total HMF removal and yeast adapted to high galactose concentrations increased the fermentation performance and decreased the fermentation time from 96 to 36 h compared to traditional fermentation.
机译：这项研究采用统计方法来获得最佳的超高温酸水解条件，该方法以金盏花（红海藻）为生物质来源。确定了以金黄色假单胞菌为生物质的最佳超高温酸水解条件，其为12％（w / v）的浆料含量，358.3 mM的H2SO4和142.6 A的温度保持11分钟。高温酸水解后，进行酶糖化。总的单糖浓度为45.1g / L，是基于120g干重/ L的金黄色葡萄球菌浆液中的总可发酵单糖62.4g / L的理论值的72.2％。为了增加乙醇产量，通过用3.5％（w / v）活性炭处理2分钟，除去水解产物中3.8 g / L的5-羟甲基糠醛（HMF），并通过单独的水解和发酵处理适应高半乳糖浓度的毕赤酵母进行发酵。 。完全去除HMF并使用适应于高半乳糖浓度的树干毕赤酵母，可生产22 g / L乙醇（收率0.50）。与传统发酵相比，完全去除HMF的发酵和适应高半乳糖浓度的酵母可提高发酵性能，并将发酵时间从96小时减少到36小时。

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