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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effects of interface adsorption of Rhodococcus ruber TH3 cells on the biocatalytic hydration of acrylonitrile to acrylamide
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Effects of interface adsorption of Rhodococcus ruber TH3 cells on the biocatalytic hydration of acrylonitrile to acrylamide

机译：红球菌TH3细胞的界面吸附对丙烯腈生物催化水合生成丙烯酰胺的影响。

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摘要

In this work, the drastic change in the reaction rate throughout the acrylonitrile bio-hydration reaction, which was catalyzed by Rhodococcus ruber TH3 free cells in a two-liquid-phase system, was studied by changing the initial mass fraction of acrylonitrile and acrylamide. We found that the reaction rate was sensitively affected by the contact area between the acrylonitrile droplets and cells. With the acrylonitrile mass fraction of 3 wt%, the cell solution of 800 U/mL could make the superficial area of acrylonitrile droplets saturated. The sustained increase of the acrylamide concentration in the reaction process could reduce the reaction rate, and 25 wt% was the obvious inflection point. The interface adsorption of cells was visually observed with the method of fluorescence microscopy, and the uptake mechanism of substrate by direct contact was illustrated to play a main role by comparing the reaction rate of the heterogeneous system and that of the homogeneous system.
机译：在这项工作中，通过改变丙烯腈和丙烯酰胺的初始质量分数，研究了由红球菌TH3无细胞在两液相体系中催化的整个丙烯腈生物水合反应中反应速率的剧烈变化。我们发现，反应速度受到丙烯腈液滴与细胞之间接触面积的敏感影响。在丙烯腈质量分数为3 wt％的情况下，800 U / mL的细胞溶液可使丙烯腈液滴的表面面积饱和。反应过程中丙烯酰胺浓度的持续增加会降低反应速率，其中25 wt％是明显的拐点。通过荧光显微镜观察肉眼观察到的细胞界面吸附，并通过比较异质系统和均相系统的反应速率，说明了直接接触底物的吸收机理。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第7期|931-938|共8页
  • 作者

    Guo Mingzhao; Yang Lufan; Li Jiahui; Jiao Song; Wang Yujun; Luo Guangsheng; Yu Huimin;

  • 作者单位

    Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China;

    Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China;

    Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China;

    Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China;

    Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China;

    Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China;

    Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Bio-hydration; Acrylonitrile; Acrylamide; Interface adsorption; Fluorescence microscopy;

    机译：生物水合丙烯腈丙烯酰胺界面吸附荧光显微镜;

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