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首页> 外文期刊>Bioprocess and Biosystems Engineering >Soluble lipase-catalyzed synthesis of methyl esters using a blend of edible and nonedible raw materials
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Soluble lipase-catalyzed synthesis of methyl esters using a blend of edible and nonedible raw materials

机译：可食用和不可食用原料的混合物，可溶脂肪酶催化合成甲酯

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摘要

This work investigates the use of blends of edible and nonedible raw materials as an alternative feedstock to fatty acid methyl esters (FAME) production through enzymatic catalysis. As biocatalyst, liquid lipase from Thermomyces lanuginosus (Callera (TM) Trans L), was used. Under reaction conditions of 35 A degrees C, methanol to feedstock molar ratio of 4.5:1 and 1.45% of catalyst load, the best process performance was reached using 9% of water concentration in the medium-yield of 79.9% after 480 min of reaction. In terms of use of tallow mixed with soybean oil, the best yield was obtained when 100% of tallow was used in the process-84.6% after 480 min of reaction-behavior that was associated with the degree of unsaturation of the feedstock, something by that time, not addressed in papers of the area. The results show that tallow can be used as an alternative to FAME production, catalyzed by soluble lipase.
机译：这项工作研究了食用和不可食用原料的混合物作为通过酶催化生产脂肪酸甲酯（FAME）的替代原料的用途。作为生物催化剂，使用了来自嗜热霉菌的液体脂肪酶（Callera TM Trans L）。在35 A的反应条件，甲醇与原料的摩尔比为4.5：1和催化剂负载的1.45％的条件下，反应480分钟后，使用9％的水浓度和79.9％的中产率，可获得最佳工艺性能。就牛油与大豆油的混合使用而言，当反应行为480分钟与原料的不饱和度有关时，在100％牛油用于过程中可获得84.6％的最佳收率。那个时候，该领域的论文中没有提到。结果表明，牛脂可以用作可溶性脂肪酶催化的FAME生产的替代品。

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