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A comparative study of eubacterial communities by PCR-DGGE fingerprints in anoxic and aerobic biotrickling filters used for biogas desulfurization

机译:厌氧和好氧生物滴滤池中用于沼气脱硫的PCR-DGGE指纹对真细菌群落的比较研究

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摘要

Biological desulfurization has proven to be a process that is technically and economically feasible on using biotrickling filters that can be performed under aerobic and anoxic conditions. However, microbial communities are different mainly due to the use of different final electron acceptors. The analysis of microbial communities in these systems has not been addressed with regard to the anoxic process. The aim of the work reported here was to analyse the eubacterial community in the two types of bioreactor along the packed bed and during the operation time. The analysis was carried out using the 16S PCR-DGGE molecular fingerprint technique. The microbial profile analysis in the aerobic bioreactor revealed that the community was more diverse and stratified compared to those obtained in the two anoxic bioreactors, influenced by environmental factors. The main OTU involved in this process is genus Thiobacillus, although different species were detected depending on each operational condition.
机译:在使用可在需氧和缺氧条件下进行的生物滴滤滤池时,生物脱硫工艺已被证明在技术上和经济上都是可行的。但是,微生物群落的不同主要是由于使用了不同的最终电子受体。关于缺氧过程,尚未解决这些系统中微生物群落的分析问题。此处报道的工作目的是分析沿填充床和运行期间两种生物反应器中的真细菌群落。使用16S PCR-DGGE分子指纹技术进行分析。有氧生物反应器中的微生物概况分析表明,与两个缺氧生物反应器中获得的那些相比,该群落更加多样化和分层,受环境因素的影响。尽管根据每个操作条件检测到不同的物种,但该过程涉及的主要OTU是硫杆菌属。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第8期|1165-1175|共11页
  • 作者单位

    Univ Cadiz, Inst Univ Invest Vitivinicola & Agroalimentario I, Dept Biomed Biotechnol & Publ Hlth Biochem & Mol, Cadiz 11510, Spain;

    Univ Cadiz, Inst Univ Invest Vitivinicola & Agroalimentario I, Fac Sci, Dept Chem Engn & Food Technol, Cadiz 11510, Spain;

    Univ Cadiz, Inst Univ Invest Vitivinicola & Agroalimentario I, Fac Sci, Dept Chem Engn & Food Technol, Cadiz 11510, Spain;

    Univ Autonoma Barcelona, Dept Chem Biol & Environm Engn, GENOCOV Res Grp, Edifici Q,Campus Bellaterra, Barcelona 08193, Spain;

    Univ Autonoma Barcelona, Dept Chem Biol & Environm Engn, GENOCOV Res Grp, Edifici Q,Campus Bellaterra, Barcelona 08193, Spain;

    Univ Cadiz, Inst Univ Invest Vitivinicola & Agroalimentario I, Fac Sci, Dept Chem Engn & Food Technol, Cadiz 11510, Spain;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    PCR-DGGE; Biotrickling filters (BTF); Anoxic hydrogen sulfide removal; Biogas; Bacterial community;

    机译:PCR-DGGE;生物滴滤池;脱氧脱硫;沼气;细菌群落;

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