• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Improved production of jiangxienone in submerged fermentation of Cordycepsjiangxiensis under nitrogen deficiency
【24h】

Improved production of jiangxienone in submerged fermentation of Cordycepsjiangxiensis under nitrogen deficiency

机译：氮不足下冬虫夏草深层发酵中江西酮的改良产量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Jiangxienone produced by Cordyceps jiangxiensis exhibits significant cytotoxicity and good selectivity against various human cancer cells, especially gastric cancer cells. In this work, the effect of nitrogen deficiency on the accumulation of jiangxienone and the transcription levels of jiangxienone biosynthesis genes was studied in submerged fermentation of C. jiangxiensis. Results showed that accumulation of jiangxienone was improved under nitrogen deficiency condition. A maximal jiangxienone content of 3.2 mu g/g cell dry weight was reached at 5 mM glutamine, and it was about 8.9-fold higher than that obtained at 60 mM glutamine (control). The transcription levels of the biosynthetic pathway genes hmgr and sqs and the nitrogen regulatory gene areA were upregulated by 7-, 14-, and 28-fold, respectively, in culture with 5 mM glutamine compared to the control. It was hypothesized that the jiangxienone biosynthesis may involve the mevalonate pathway in C. jiangxiensis. Taken together, our study indicated that nitrogen deficiency is an efficient strategy for enhancing jiangxienone accumulation in submerged fermentation of C. jiangxiensis, which is useful for further understanding the regulation of jiangxienone biosynthesis.
机译：江西冬虫夏草产生的江西烯酮对多种人类癌细胞，尤其是胃癌细胞具有明显的细胞毒性和良好的选择性。在这项工作中，研究了氮不足对江西海藻深层发酵中江西烯酮积累和江西烯酮生物合成基因转录水平的影响。结果表明，在缺氮条件下，江西烯酮的积累得到改善。在5 mM谷氨酰胺中，江西烯酮的最大含量为3.2干克/克细胞干重，比60 mM谷氨酰胺（对照）时高约8.9倍。与对照相比，在含有5 mM谷氨酰胺的培养物中，生物合成途径基因hmgr和sqs和氮调节基因areA的转录水平分别上调了7倍，14倍和28倍。假设江西烯酮的生物合成可能与江西梭子甲的甲羟戊酸途径有关。综上所述，我们的研究表明氮缺乏是提高江西克雷氏菌深层发酵中江西烯酮积累的有效策略，对于进一步了解江西烯酮的生物合成调控是有用的。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2018年第10期|1417-1423|共7页
  • 作者

    Jiang Lu-Xi; Han Li-Liang; Wang Hui-Ping; Xu Jun-Wei; Xiao Jian-Hui;

  • 作者单位

    Zunyi Med Univ, Affiliated Hosp, Inst Med Biotechnol, Div Appl Mycol & Biochem Pharm, Zunyi 563000, Peoples R China;

    Zunyi Med Univ, Affiliated Hosp, Inst Med Biotechnol, Div Appl Mycol & Biochem Pharm, Zunyi 563000, Peoples R China;

    Kunming Med Univ, Kunming Childrens Hosp, Dept Neurol, Kunming 650228, Yunnan, Peoples R China;

    Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China;

    Zunyi Med Univ, Affiliated Hosp, Inst Med Biotechnol, Div Appl Mycol & Biochem Pharm, Zunyi 563000, Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Jiangxienone; Cordyceps jiangxiensis; Nitrogen deficiency; Biosynthetic gene;

    机译：江西烯酮;江西冬虫夏草;氮缺乏;生物合成基因;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号