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首页> 外文期刊>Bioprocess and Biosystems Engineering >Feasibility of attached cultivation for polysaccharides production by Porphyridium cruentum
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Feasibility of attached cultivation for polysaccharides production by Porphyridium cruentum

机译：附着培养法生产克鲁氏卟啉单胞菌多糖的可行性

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摘要

Porphyridium cruentum is one of the most valued microalgae species able to produce both pigments and exopolysaccharides. Conventional liquid suspended cultivation in ponds and photobioreactors show its disadvantages in lower cultivation efficiency and higher stirring power consumption due to the high viscosity of the medium by the accumulation of polysaccharides. In this work, a new method of culture (called attached cultivation) based on the growth of microalgae using a supporting surface was successfully applied to the cultivation of P. cruentum and the effect of the main influential parameters on its growth rate and polysaccharides production has been investigated. Higher values of these factors resulted in a faster growth rate and, in particular, optimum values of 6.98 g m(-2) for initial biomass density, 100 A mu mol m(-2) s(-1) for light intensity, continuous illumination, 2.0 % for CO2 concentration, and 0.1 v v(-1) min(-1) for aeration rate produced the best polysaccharide production of 42 % dry weight. The nutrition profile of P. cruentum obtained in attached and suspended cultivations was similar. Overall these results demonstrate that the attached cultivation is a promising technique which greatly improves the growth rate of P. cruentum as well as its production of polysaccharides and, therefore, it is worth enhancing to be exploited for commercial application.
机译：猪角卟啉是最有价值的微藻物种之一，能够同时产生色素和胞外多糖。在池塘和光生物反应器中的常规液体悬浮培养显示出其缺点，即由于多糖的积累导致培养基的高粘度，因此导致较低的培养效率和较高的搅拌功率消耗。在这项工作中，基于微藻生长的一种新的培养方法（称为附着培养）被成功地应用于了克鲁氏假单胞菌的培养，该方法利用支撑表面生长微藻，并且主要的影响参数对其生长速率和多糖产量产生了影响。被调查。这些因子的值越高，生长速度越快，特别是对于初始生物量密度，最佳值为6.98 gm（-2），对于光强度，连续照明，最佳值为100 Aμmol m（-2）s（-1） ，CO2浓度为2.0％，通气速率为0.1vv（-1）min（-1）时，干重为42％的多糖产量最高。在附着和悬浮培养中获得的景天体育的营养状况相似。总的来说，这些结果表明，附着栽培是一种有前途的技术，它可以极大地提高景天螺的生长速度以及其多糖的产生，因此，值得推广用于商业应用。

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