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首页> 外文期刊>Bioprocess and Biosystems Engineering >Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis
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Cellulases immobilization on chitosan-coated magnetic nanoparticles: application for Agave Atrovirens lignocellulosic biomass hydrolysis

机译：纤维素酶固定在壳聚糖包被的磁性纳米颗粒上：在龙舌兰Atrovirens木质纤维素生物质水解中的应用

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摘要

In the present study, Trichoderma reesei cellulase was covalently immobilized on chitosan-coated magnetic nanoparticles using glutaraldehyde as a coupling agent. The average diameter of magnetic nanoparticles before and after enzyme immobilization was about 8 and 10 nm, respectively. The immobilized enzyme retained about 37 % of its initial activity, and also showed better thermal and storage stability than free enzyme. Immobilized cellulase retained about 80 % of its activity after 15 cycles of carboxymethylcellulose hydrolysis and was easily separated with the application of an external magnetic field. However, in this reaction, K (m) was increased eight times. The immobilized enzyme was able to hydrolyze lignocellulosic material from Agave atrovirens leaves with yield close to the amount detected with free enzyme and it was re-used in vegetal material conversion up to four cycles with 50 % of activity decrease. This provides an opportunity to reduce the enzyme consumption during lignocellulosic material saccharification for bioethanol production.
机译：在本研究中，使用戊二醛作为偶联剂，将里氏木霉纤维素酶共价固定在壳聚糖包被的磁性纳米颗粒上。酶固定前后磁性纳米颗粒的平均直径分别约为8和10 nm。固定化酶保留了其初始活性的约37％，并且还显示出比游离酶更好的热稳定性和储存稳定性。在羧甲基纤维素水解15个循环后，固定的纤维素酶保留了约80％的活性，并且在施加外部磁场的情况下很容易分离。然而，在该反应中，K（m）增加了八倍。固定化酶能够水解龙舌兰叶片的木质纤维素材料，收率接近游离酶检测到的量，并且可用于植物材料转化，最多重复使用四个周期，活性降低50％。这提供了减少用于生物乙醇生产的木质纤维素材料糖化过程中酶消耗的机会。

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