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首页> 外文期刊>Bioprocess and Biosystems Engineering >Cellotriose-hydrolyzing activity conferred by truncating the carbohydrate-binding modules of Cel5 from Hahella chejuensis
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Cellotriose-hydrolyzing activity conferred by truncating the carbohydrate-binding modules of Cel5 from Hahella chejuensis

机译：截短中华绒螯蟹Cel5的碳水化合物结合模块而赋予的纤维素三糖水解活性

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摘要

Processivity is a typical characteristic of cellobiohydrolases (CBHs); it enables the enzyme to successively hydrolyze the ends of cellulose chains and to produce cellobiose as the major product. Some microbes, which do not have CBHs, utilize endoglucanases (EGs) that exhibit processivity, commonly referred to as processive EGs. A processive EG identified from Hahella chejuensis, HcCel5, has a catalytic domain (CD) belonging to the glycoside hydrolase family 5 (GH5) and two carbohydrate- binding modules (CBM6s). In this study, we compared HcCel5-CD with the CD of Saccharophagus degradans Cel5H (SdCel5H-CD), which is a processive EG reported previously. Our results showed that in comparison to SdCel5H-CD, HcCel5-CD has more suitable characteristics for cellulose hydrolysis, such as higher hydrolytic activity, thermostability (40-80 degrees C), and processivity. Noticeably, HcCel5-CD is capable of hydrolyzing cellotriose, unlike HcCel5. These features of HcCel5-CD for cellulose hydrolysis could be employed for efficient saccharification of lignocellulose to produce cellobiose and glucose, which may be used to produce renewable fuels and chemicals.
机译：连续性是纤维二糖水解酶（CBHs）的典型特征。它使酶能够连续水解纤维素链的末端并产生纤维二糖作为主要产物。一些没有CBH的微生物利用表现出可生产性的内切葡聚糖酶（EG），通常称为生产性EG。从Hahella chejuensis HcCel5鉴定出的进行性EG具有属于糖苷水解酶家族5（GH5）的催化域（CD）和两个碳水化合物结合模块（CBM6s）。在这项研究中，我们比较了HcCel5-CD和Saccharophagus degradans Cel5H（SdCel5H-CD）的CD，这是先前报道的一种进行性EG。我们的结果表明，与SdCel5H-CD相比，HcCel5-CD具有更适合纤维素水解的特性，例如更高的水解活性，热稳定性（40-80摄氏度）和可加工性。值得注意的是，与HcCel5不同，HcCel5-CD能够水解纤维三糖。 HcCel5-CD用于纤维素水解的这些特征可用于木质纤维素的有效糖化以生产纤维二糖和葡萄糖，可用于生产可再生燃料和化学物质。

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