• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea
【24h】

Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea

机译：通过mutB基因敲除阻止丙酸酯流入TCA循环会导致工业红细菌性红多孢菌（Saccharopolyspora erythraea）产生红霉素的大量增加

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A high erythromycin producing mutant strain Saccharopolyspora erythraea HL3168 E3-Delta mutB was constructed by deleting mutB (SACE_5639) gene encoding the beta subunit of methylmalonyl-CoA mutase of an industrial strain of S. erythraea HL3168 E3. Industrial media and process control strategies were adopted in a 5 L bioreactor for characterizing the physiological parameters. The total erythromycin titer and erythromycin A concentration in mutant were 46.9 (12740.5 mu g/mL) and 64.9 % (8094.4 mu g/mL) higher than those in original strain, respectively, which were comparable to industrial erythromycin production. The specific glucose and n-propanol consumption rates were increased by 52.4 and 39.8 %, respectively. During the rapid erythromycin synthesis phase, the yield of erythromycin on n-propanol also increased from 24.3 % in control group to 66.9 % in mutant group. Meanwhile, the specific formation rates of methylmalonyl-CoA and propionyl-CoA, two crucial precursors for erythromycin synthesis, were 1.89-and 2.02folds higher in the mutant strain, respectively.
机译：通过缺失编码红球菌HL3168 E3工业菌株的甲基丙二酰-CoA变位酶β亚基的mutB（SACE_5639）基因，构建了高产红霉素突变株红球菌HL3168 E3-Delta mutB。在5 L生物反应器中采用工业介质和过程控制策略来表征生理参数。突变体中的红霉素总滴度和红霉素A浓度分别比原始菌株高46.9（12740.5μg / mL）和64.9％（8094.4μg / mL），可与工业红霉素生产相媲美。葡萄糖和正丙醇的比消耗率分别增加了52.4％和39.8％。在快速的红霉素合成阶段，红霉素在正丙醇上的产率也从对照组的24.3％增加到突变组的66.9％。同时，在突变菌株中，甲基丙二酰辅酶A和丙酰辅酶A（红霉素合成的两个关键前体）的比形成速率分别高1.89倍和2.02倍。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2017年第2期|201-209|共9页
  • 作者

    Chen Chongchong; Hong Ming; Chu Ju; Huang Mingzhi; Ouyang Liming; Tian Xiwei; Zhuang Yingping;

  • 作者单位

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;

    East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Saccharopolyspora erythraea; Erythromycin; N-propanol; Methylmalonyl-CoA mutase; mutB;

    机译：糖多孢菌;红霉素;N-丙醇;甲基丙二酰辅酶A变位酶;mutB;

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号