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Strategies on process engineering of chondrocyte culture for cartilage tissue regeneration

机译：软骨组织再生软骨细胞培养过程工程的策略

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The current work is an attempt to study the strategies for cartilage tissue regeneration using porous scaffold in wavy walled airlift bioreactor (ALBR). Novel chitosan, poly (l-lactide) and hyaluronic acid based composite scaffold were prepared. The scaffolds were cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide and chondroitin sulfate to obtain interconnected 3D microstructure showing excellent biocompatibility, higher cellular differentiation and increased stability. The surface morphology and porosity of the scaffolds were analyzed using scanning electron microscopy (SEM) and mercury intrusion porosimeter and optimized for chondrocyte regeneration. The study shows that the scaffolds were highly porous with pore size ranging from 48 to 180 A mu m and the porosities in the range 80-92%. Swelling and in vitro degradation studies were performed for the composite scaffolds; by increasing the chitosan: HA ratio in the composite scaffolds, the swelling property increases and stabilizes after 24 h. There was controlled degradation of composite scaffolds for 4 weeks. The uniform chondrocyte distribution in the scaffold using various growth modes in the shake flask and ALBR was studied by glycosaminoglycans (GAG) quantification, MTT assay and mixing time evaluation. The cell culture studies demonstrated that efficient designing of ALBR increases the cartilage regeneration as compared to using a shake flask. The free chondrocyte microscopy and cell attachment were performed by inverted microscope and SEM, and from the study it was confirmed that the cells uniformly attached to the scaffold. This study focuses on optimizing strategies for the culture of chondrocyte using suitable scaffold for improved cartilage tissue regeneration.
机译：当前的工作是尝试研究波浪壁空运生物反应器（ALBR）中使用多孔支架的软骨组织再生策略。制备了新型的壳聚糖，聚（丙交酯）和透明质酸基复合支架。支架与1-乙基-3-（3-二甲基氨基丙基）碳二亚胺，N-羟基琥珀酰亚胺和硫酸软骨素交联，以获得相互连接的3D微观结构，显示出出色的生物相容性，更高的细胞分化能力和更高的稳定性。使用扫描电子显微镜（SEM）和压汞仪测量支架的表面形态和孔隙率，并优化软骨细胞的再生。研究表明，支架高度多孔，孔径范围为48至180 Aμm，孔隙率范围为80-92％。对复合支架进行了溶胀和体外降解研究。通过增加复合支架中壳聚糖：HA的比例，溶胀性增加并在24小时后稳定。复合支架的降解被控制了4周。通过糖胺聚糖（GAG）定量，MTT分析和混合时间评估，研究了摇瓶和ALBR中各种生长模式下支架中软骨细胞的均匀分布。细胞培养研究表明，与使用摇瓶相比，有效设计ALBR可提高软骨再生。通过倒置显微镜和SEM进行游离软骨细胞显微镜检查和细胞附着，并且从研究中证实细胞均匀地附着在支架上。这项研究的重点是使用合适的支架优化软骨组织再生的软骨细胞培养策略。

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