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Innovative methods to generate clean sugar stream from biomass feedstocks for efficient fermentation

机译:从生物质原料产生清洁糖流以进行有效发酵的创新方法

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Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural (HMF) and furfural, were effectively removed using an appropriate detoxification method using 1% (w/v) activated charcoal. The resin mixture containing cationic exchangers (DOWEX 50WX8) and anionic exchangers (IRA 743) in 7:3 ratio at pH 2.7 completely removed HMF, acetic acid and formic acid with less than 2% (w/w) sugar loss, leading to efficient detoxification of inhibitory compounds.
机译:对高粱秸秆进行了评估,以产生纯净的糖分;与碱法(49.1 g糖/ g预处理生物质)相比,酸预处理的生物质的酶促水解效率极低(12.3 g糖/ g预处理生物质)。使用适当的解毒方法,使用1%(w / v)活性炭,有效地去除了酸预处理产生的抑制性化合物,例如羟甲基糠醛(HMF)和糠醛。在pH值为2.7的比例为7:3的阳离子交换剂(DOWEX 50WX8)和阴离子交换剂(IRA 743)的树脂混合物中,糖分损失少于2%(w / w)的HMF,乙酸和甲酸被完全去除,从而实现了高效排毒抑制性化合物。

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