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首页> 外文期刊>Bioprocess and Biosystems Engineering >Metabolic engineering of Synechocystis sp PCC 6803 for enhanced ethanol production based on flux balance analysis
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Metabolic engineering of Synechocystis sp PCC 6803 for enhanced ethanol production based on flux balance analysis

机译：基于通量平衡分析的集胞藻PCC 6803的代谢工程，可提高乙醇产量

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Synechocystis sp. PCC 6803 is an attractive host for bio-ethanol production due to its ability to directly convert atmospheric carbon dioxide into ethanol using photosystems. To enhance ethanol production in Synechocystis sp. PCC 6803, metabolic engineering was performed based on in silico simulations, using the genome-scale metabolic model. Comprehensive reaction knockout simulations by flux balance analysis predicted that the knockout of NAD(P)H dehydrogenase enhanced ethanol production under photoautotrophic conditions, where ammonium is the nitrogen source. This deletion inhibits the re-oxidation of NAD(P)H, which is generated by ferredoxin-NADP(+) reductase and imposes re-oxidation in the ethanol synthesis pathway. The effect of deleting the ndhF1 gene, which encodes NADH dehydrogenase subunit 5, on ethanol production was experimentally evaluated using ethanol-producing strains of Synechocystis sp. PCC 6803. The ethanol titer of the ethanol-producing a dagger ndhF1 strain increased by 145%, compared with that of the control strain.
机译：集胞藻PCC 6803是生产生物乙醇的诱人主机，因为它具有使用光系统将大气中的二氧化碳直接转化为乙醇的能力。为了提高集胞藻属中的乙醇产量。使用基因组规模的代谢模型，基于计算机模拟，进行了PCC 6803代谢工程。通过通量平衡分析进行的综合反应敲除模拟预测，在铵盐为氮源的光合自养条件下，NAD（P）H脱氢酶的敲除可提高乙醇产量。这种删除抑制了由铁氧还蛋白-NADP（+）还原酶产生的NAD（P）H的再氧化，并在乙醇合成途径中施加了再氧化。使用产乙醇的Synechocystis sp。菌株，通过实验评估了删除编码NADH脱氢酶亚基5的ndhF1基因对乙醇生产的影响。 PCC6803。与对照菌株相比，生产匕首ndhF1菌株的乙醇的乙醇滴度提高了145％。

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